The c-Myc-regulated lncRNA NEAT1 and paraspeckles modulate imatinib-induced apoptosis in CML cells

Molecular Cancer
Chengwu ZengYangqiu Li

Abstract

Chronic myeloid leukemia (CML) is a clonal disease characterized by the presence of the constitutively active tyrosine kinase BCR-ABL oncoprotein. Although BCR-ABL is crucially important for pathogenesis and treatment response, it is thought that some additional factors might be involved in the regulation of these processes. Aberrant expression of long noncoding RNAs (lncRNAs) has recently been identified to be involved in various diseases including cancer, suggesting that lncRNAs may play a role in BCR-ABL-mediated CML. In this study, we found that nuclear-enriched abundant transcript 1 (NEAT1), a lncRNA essential for the formation of nuclear body paraspeckles, is significantly repressed in primary CML cells. NEAT1 expression could be restored by inhibiting BCR-ABL expression or its kinase activity in K562 cells. We also demonstrated that NEAT1 is regulated by c-Myc. Knockdown of NEAT1 could promote imatinib (IM)-induced apoptosis, and we demonstrated that the NEAT1-binding paraspeckle protein splicing factor proline/glutamine-rich (SFPQ) is required for NEAT1-mediated apoptosis in K562 cells. RNA-seq analysis revealed that SFPQ regulates cell growth and death pathway-related genes, confirming its function in IM-induced apopto...Continue Reading

References

Mar 7, 2003·Cancer Cell·Rossana TrottaBruno Calabretta
Nov 1, 2013·Molecular Biology of the Cell·Tetsuro HiroseGérard Pierron
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May 10, 2018·Journal of Hematology & Oncology·Nicola AmodioPierfrancesco Tassone

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Citations

Jun 6, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Jingyi YanWei Chen
Jul 3, 2019·Asia-Pacific Journal of Clinical Oncology·Xibao YuYixin Zeng
Jan 15, 2020·Cancer Control : Journal of the Moffitt Cancer Center·Dongdong LiuGuobiao Liang
Sep 30, 2020·Omics : a Journal of Integrative Biology·Hilal Taymaz-NikerelBetül Kırdar
Jun 19, 2020·International Journal of Molecular Sciences·Eleonora VecchioIleana Quinto
Jun 28, 2019·Cell Death & Disease·Shanshan WangBo Zuo
Mar 29, 2020·Cellular and Molecular Life Sciences : CMLS·Ziqiang WangWeiren Huang
Jul 6, 2020·Annals of Hematology·Leonidas BenetatosGeorgios Vartholomatos
Nov 4, 2020·ELife·Shan JiangBeidong Liu
Dec 10, 2020·Cancers·Subhasree KumarJean-Pierre Etchegaray
Nov 3, 2020·Frontiers in Oncology·Kaifee Arman, Tarik Möröy
Nov 5, 2020·Non-coding RNA·Gabriel Pisani, Byron Baron
Dec 9, 2020·Molecular and Cellular Biology·Deena BaderNiketa A Patel
Nov 8, 2020·Seminars in Cancer Biology·Rongfu TuGuoliang Qing
Oct 4, 2020·Pathology, Research and Practice·Yun-Ya LuoYun-Xiao Xu
Jun 24, 2021·Asia-Pacific Journal of Clinical Oncology·Chunsheng FangChengwu Zeng
Aug 21, 2021·Frontiers in Cell and Developmental Biology·Nonthaphat Kent WongChien-Ling Huang
Aug 28, 2021·Biomedicines·Ekaterina Mikhailovna StasevichAnton Markovich Schwartz

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Methods Mentioned

BETA
RNA-seq
transfection
ChIP
PCR
flow cytometry
confocal microscopy

Software Mentioned

DAVID Bioinformatics

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