The C-terminal domain of B-Myb acts as a positive regulator of transcription and modulates its biological functions.

Molecular and Cellular Biology
I H Oh, E P Reddy

Abstract

The myb gene family consists of three members, named A-, B-, and c-myb. All three members of this family encode nuclear proteins that bind DNA in a sequence-specific manner and function as regulators of transcription. In this report, we have examined the biochemical and biological activities of murine B-myb and compared these properties with those of murine c-myb. In transient transactivation assays, murine B-myb exhibited transactivation potential comparable to that of c-myb. An analysis of deletion mutants of B-myb and c-myb showed that while the C-terminal domain of c-Myb acts as a negative regulator of transcriptional transactivation, the C-terminal domain of B-Myb functions as a positive enhancer of transactivation. To compare the biological activities of c-myb and B-myb, the two genes were overexpressed in 32Dcl3 cells, which are known to undergo terminal differentiation into granulocytes in the presence of granulocyte colony-stimulating factor (G-CSF). We observed that c-myb blocked the G-CSF-induced terminal differentiation of 32Dcl3 cells, resulting in their continued proliferation in the presence of G-CSF. In contrast, ectopic overexpression of B-myb blocked the ability of 32D cells to proliferate in the presence of G...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H DudekE P Reddy
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P SaikumarE P Reddy
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H SakuraS Ishii
Feb 1, 1988·Molecular and Cellular Biology·M F ClarkeE V Prochownik
Dec 9, 1988·Nucleic Acids Research·N NomuraR Ishizaki
Oct 27, 1988·Nature·H BiedenkappK H Klempnauer
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K TodokoroY Ikawa
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R G RamsayP A Marks
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S GreenbergerR J Eckner
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E H WestinF Wong-Staal
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·N AzizT P Bender
Jan 1, 1994·Critical Reviews in Oncogenesis·J LyonR Watson
Jan 25, 1993·Nucleic Acids Research·R J WatsonE W Lam
Mar 1, 1996·Genes & Development·P DaiS Ishii
Apr 1, 1996·Molecular and Cellular Biology·S MinkK H Klempnauer

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Citations

May 29, 2002·Oncogene·Jiang Zhu, Stephen G Emerson
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Clement M LeeE Premkumar Reddy
Dec 26, 2001·The Journal of Biological Chemistry·Lance R JohnsonAngie Rizzino
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Keelan Z GuileySeth M Rubin
Nov 14, 2000·The Journal of Biological Chemistry·Y Sano, S Ishii
Sep 25, 1999·The Journal of Biological Chemistry·Y TanakaS Ishii
Jan 7, 2000·Experimental Cell Research·A EngelhardB Calabretta
Feb 5, 2005·Developmental Cell·Mark L SandbergMichael P Cooke

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