The C-terminal extension of ferrochelatase is critical for enzyme activity and for functioning of the tetrapyrrole pathway in Synechocystis strain PCC 6803

Journal of Bacteriology
Roman SobotkaMartin Tichý

Abstract

Heme and chlorophyll (Chl) share a common biosynthetic pathway up to the branch point where magnesium chelatase and ferrochelatase (FeCH) insert either magnesium for Chl biosynthesis or ferrous iron for heme biosynthesis. A distinctive feature of FeCHs in cyanobacteria is their C-terminal extension, which forms a putative transmembrane segment containing a Chl-binding motif. We analyzed the deltaH324 strain of Synechocystis sp. strain PCC 6803, which contains a truncated FeCH enzyme lacking this C-terminal domain. Truncated FeCH was localized to the membrane fraction, suggesting that the C-terminal domain is not necessary for membrane association of the enzyme. Measurements of enzyme activity and complementation experiments revealed that the deltaH324 mutation dramatically reduced activity of the FeCH, which resulted in highly upregulated 5-aminolevulinic acid synthesis in the deltaH324 mutant, implying a direct role for heme in the regulation of flux through the pathway. Moreover, the deltaH324 mutant accumulated a large amount of protoporphyrin IX, and levels of Chl precursors were also significantly increased, suggesting that some, but not all, of the "extra" flux can be diverted down the Chl branch. Analysis of the recombin...Continue Reading

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Citations

Jul 3, 2010·Photosynthesis Research·Jonathan A D Neilson, Dion G Durnford
Oct 4, 2011·The Journal of Biological Chemistry·Elvira Olmedo-VerdIgnacio Luque
Jul 16, 2008·The Journal of Biological Chemistry·Roman SobotkaAnnegret Wilde
Jul 5, 2012·Journal of Biotechnology·Otilia CheregiChristiane Funk
Apr 5, 2011·Biochimica Et Biophysica Acta·Christiane FunkBeverley R Green
Sep 13, 2011·Biochimica Et Biophysica Acta·Lan-Xin ShiWolfgang P Schröder
Mar 27, 2010·Annals of Botany·Peter J NixonJosef Komenda
Jan 27, 2017·Microbiology and Molecular Biology Reviews : MMBR·Harry A DaileyMartin J Warren
Apr 4, 2020·The Journal of Biological Chemistry·Donald A BryantMartin J Warren
Dec 16, 2016·G3 : Genes - Genomes - Genetics·Miguel A Hernández-PrietoMin Chen
Feb 4, 2012·The Arabidopsis Book·Ryouichi TanakaTatsuru Masuda

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