PMID: 11606196Oct 19, 2001Paper

The C-terminal half of Hsp90 is responsible for its cytoplasmic localization

European Journal of Biochemistry
S PassinenT Ylikomi

Abstract

With some exceptions, research so far has shown heat shock protein (Hsp) 90 to be a cytoplasmic protein. Here, we studied the sequence determinants which dictate the subcellular localization of Hsp90. By constructing hybrid molecules between a nuclear protein, progesterone receptor (PR), and parts of Hsp90, we demonstrated that the C-terminal but not the N-terminal half of Hsp90 can prevent nuclear translocation of the PR. Studies with an antibody raised against a region which contains the major nuclear localization signal (NLS) of the PR suggest that the inhibition of nuclear localization is not due to steric hindrance of the NLS of the PR by Hsp90 sequences in hybrid molecules. In order to characterize further the cytoplasmic anchoring of Hsp90 we constructed four chimeric molecules between the C-terminal half of Hsp90 and estrogen receptor (ER) with different numbers of nuclear localization protosignals (proto-NLS). When the C-terminal half of Hsp90 was fused with ER containing no or one proto-NLS, the hybrid molecule was located exclusively in the cytoplasm. When the nuclear translocation signal was strengthened by adding two or three protosignals, the hybrid molecule was exclusively nuclear. These results suggest that the ...Continue Reading

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Citations

Mar 10, 2010·Amino Acids·Wiktor Prus, Anna Filipek
May 24, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Tommi ManninenTimo Ylikomi
Mar 25, 2006·Future Oncology·Georgios V Georgakis, Anas Younes
Aug 26, 2011·The International Journal of Biochemistry & Cell Biology·Wiktor PrusAnna Filipek
Mar 25, 2006·Expert Opinion on Pharmacotherapy·Luis Fayad, Anas Younes
Jan 28, 2006·FEMS Microbiology Reviews·James P BurnieRuth C Matthews
Mar 11, 2018·Scientific Reports·Kanjana SrisutthisamphanDuncan R Smith
Jan 1, 2013·Brain Sciences·Araceli Espinosa-JeffreyJean de Vellis
Dec 8, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rongmin Zhao, Walid A Houry
Jan 23, 2020·International Journal of Molecular Sciences·Magdalena Gorska-PonikowskaFrancesco Cappello
Mar 20, 2019·Nature Cell Biology·Suyong ChoiRichard A Anderson
Jan 31, 2007·Biochemistry·Graciela Piwien PilipukMario D Galigniana

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