The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human

PLoS Genetics
Katharina HannigJoachim Griesenbeck

Abstract

RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse organisms, the molecular basis of the robust rRNA production in vivo remains largely unknown. In S. cerevisiae, the multifunctional Net1 protein was reported to stimulate Pol I transcription. We found that the Pol I-stimulating function can be attributed to the very C-terminal region (CTR) of Net1. The CTR was required for normal cell growth and Pol I recruitment to rRNA genes in vivo and sufficient to promote Pol I transcription in vitro. Similarity with the acidic tail region of mammalian Pol I transcription factor UBF, which could partly functionally substitute for the CTR, suggests conserved roles for CTR-like domains in Pol I transcription from yeast to human.

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Citations

Mar 7, 2020·Nature Communications·Michael Pilsl, Christoph Engel

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Methods Mentioned

BETA
fluorescence microscopy
Immuno-Precipitation
ChIP
PCR
electrophoresis
tandem affinity purification
ChEC
in vitro transcription

Software Mentioned

ImageJ
EMBOSS
Gene 6000
Net1
HH
Gene
Rotor
- Job Dispatcher framework
EMBL
suite

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