PMID: 9421501Feb 28, 1998Paper

The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision

Nucleic Acids Research
G MoolenaarN Goosen

Abstract

The incisions in the DNA at the 3'- and 5'-side of a DNA damage during nucleotide excision repair in Escherichia coli occur in a complex consisting of damaged DNA, UvrB and UvrC. The exact requirements for the two incision events, however, are different. It has previously been shown that the 3'-incision requires the interaction between the C-terminal domain of UvrB and a homologous region in UvrC. This interaction, however, is dispensable for the 5'-incision. Here we show that the C-terminal domain of the UvrC protein is essential for the 5'-incision, whereas this domain can be deleted without affecting the 3'-incision. The C-terminal domain of UvrC is homologous with the C-terminal part of the ERCC1 protein which, in a complex with XPF, is responsible for the 5'-incision reaction in human nucleotide excision repair. Both in the UvrC and the ERCC1 domain a Helix-hairpin-Helix (HhH) motif can be indicated, albeit at different positions. Such a motif also has been found in a large variety of DNA binding proteins and it has been suggested to form a structure involved in non-sequence-specific DNA binding. In contrast to the full length UvrC protein, a truncated UvrC protein (UvrC554) lacking the entire ERCC1 homology including the ...Continue Reading

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Citations

Jun 2, 2005·Mutation Research·Bennett Van HoutenCaroline Kisker
Sep 2, 1998·Nucleic Acids Research·W L de LaatJ H Hoeijmakers
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Geri F MoolenaarNora Goosen
Feb 5, 2005·The EMBO Journal·James J TruglioCaroline Kisker
Dec 18, 1998·The Journal of Biological Chemistry·G F MoolenaarN Goosen
Apr 2, 2004·The Journal of Biological Chemistry·Irene Gómez-PintoCarlos González
Oct 23, 2016·Journal of Bacteriology·Anthonige Vidya PereraJustin Courcelle
Nov 1, 2000·The Journal of Biological Chemistry· Tang MsY Y Tang
Nov 20, 2003·The Journal of Biological Chemistry·Alexandre A Vetcher, Robert D Wells
Oct 17, 2007·Molecular and Cellular Biology·Brian S GarrisonMark A Kay
Feb 9, 2006·Chemical Reviews·James J TruglioCaroline Kisker
Feb 1, 2013·EcoSal Plus·Sophie CouvéMurat Saparbaev

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