PMID: 9539723May 16, 1998Paper

The C terminus of beta-tubulin regulates vinblastine-induced tubulin polymerization

Proceedings of the National Academy of Sciences of the United States of America
S S Rai, J Wolff

Abstract

Oligoanions such as sodium triphosphate or GTP prevent and/or reverse vinblastine-induced polymerization of tubulin. We now show that the anions of glutamate-rich extreme C termini of tubulin are similarly involved in the regulation of the vinblastine effect. Cleavage of the C termini by limited proteolysis with subtilisin enhances vinblastine-induced tubulin polymerization and abolishes the anion effect. Only the beta-tubulin C terminus needs to be removed to achieve these changes and the later cleavage of the alpha-tubulin C terminus has little additional effect. In fact, vinblastine concentrations >20 microM block cleavage of the alpha-tubulin C terminus in the polymer, whereas cleavage of the beta-tubulin C terminus proceeds unimpeded over the time used. The vinblastine effect on tubulin polymerization is also highly pH-dependent between pH 6.5 and 7.5; this is less marked, but not absent, after subtilisin treatment. A working model is proposed wherein an anionic domain proximal to the extreme C terminus must interact with a cationic domain to permit vinblastine to promote polymerization. Both exogenous and extreme C-terminal anions compete for the cationic domain with the proximal anionic domain to prevent vinblastine-indu...Continue Reading

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Citations

May 26, 1999·Cell Motility and the Cytoskeleton·L KniplingJ Wolff
May 8, 2014·Journal of Experimental Botany·Elisabeth Lipka, Sabine Müller
Sep 10, 2014·International Journal of Molecular Sciences·Isabelle L Di MaïoVincent Peyrot
Aug 31, 2000·Annual Review of Biochemistry·E Nogales
Jul 10, 2001·Annual Review of Biophysics and Biomolecular Structure·E Nogales
May 15, 2013·The Plant Journal : for Cell and Molecular Biology·Diego BreviarioLaura Morello
Jun 6, 2020·Analytical Biochemistry·Colleen McClungCristian I Ruse
Feb 14, 2016·Life Sciences·Mary Elizabeth Gnanambal K, Shailaja Vommi Lakshmipathy
Feb 1, 2006·Biochemical and Biophysical Research Communications·Leslie Knipling, J Wolff

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