The C terminus of foamy retrovirus Gag contains determinants for encapsidation of Pol protein into virions.

Journal of Virology
Eun-Gyung Lee, Maxine L Linial

Abstract

Foamy viruses (FV) differ from orthoretroviruses in many aspects of their replication cycle. A major difference is in the mode of Pol expression, regulation, and encapsidation into virions. Orthoretroviruses synthesize Pol as a Gag-Pol fusion protein so that Pol is encapsidated into virus particles through Gag assembly domains. However, as FV express Pol independently of Gag from a spliced mRNA, packaging occurs through a distinct mechanism. FV genomic RNA contains cis-acting sequences that are required for Pol packaging, suggesting that Pol binds to RNA for its encapsidation. However, it is not known whether Gag is directly involved in Pol packaging. Previously our laboratory showed that sequences flanking the three glycine-arginine-rich (GR) boxes at the C terminus of FV Gag contain domains important for RNA packaging and Pol expression, cleavage, and packaging. We have now shown that both deletion and substitution mutations in the first GR box (GR1) prevented neither the assembly of particles with wild-type density nor packaging of RNA genomes but led to a defect in Pol packaging. Site-directed mutagenesis of GR1 indicated that the clustered positively charged amino acids in GR1 play important roles in Pol packaging. Our res...Continue Reading

References

Aug 1, 1994·Journal of Virology·A W Schliephake, A Rethwilm
Jan 1, 1996·Current Topics in Microbiology and Immunology·R BerkowitzS P Goff
Sep 1, 1996·Journal of Viral Hepatitis·M Nassal, H Schaller
Aug 28, 1997·Biochemical and Biophysical Research Communications·K I PfrepperR M Flügel
Jan 28, 1998·Journal of Virology·N FischerA Rethwilm
Apr 29, 1998·Journal of Virology·D N Baldwin, M L Linial
Jan 9, 1999·Journal of Virology·S F YuM L Linial
Nov 22, 2000·The Journal of General Virology·H ImrichA Rethwilm
Oct 15, 2003·Journal of Virology·Jacqueline RoyAxel Rethwilm
Aug 17, 2004·Journal of Virology·Carolyn R Stenbak, Maxine L Linial
Mar 9, 2007·Journal of Virology·Jacqueline Roy, Maxine L Linial

❮ Previous
Next ❯

Citations

May 7, 2010·Medical Microbiology and Immunology·Axel Rethwilm
Jul 16, 2010·Gene Therapy·O Erlwein, M O McClure
Feb 18, 2011·Journal of Virology·Maximilian J HartlBirgitta M Wöhrl
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Mar 23, 2013·Viruses·Eun-Gyung LeeMaxine L Linial
Jul 23, 2013·Viruses·Md Alamgir HossainCha-Gyun Shin
Jan 15, 2009·The Journal of General Virology·Tatiana WiktorowiczAxel Rethwilm
Jul 4, 2012·Mobile Genetic Elements·Yury StefanovIvan Glukhov
Jul 20, 2010·Cell Host & Microbe·Johan A den BoonPaul Ahlquist
Sep 3, 2016·Viruses·Martin V Hamann, Dirk Lindemann
Jan 7, 2017·Virus Evolution·Ryan Ruboyianes, Michael Worobey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.