The C-terminus of IcmT is essential for pore formation and for intracellular trafficking of Legionella pneumophila within Acanthamoeba polyphaga

Molecular Microbiology
Maëlle MolmeretYousef Abu Kwaik

Abstract

We have shown previously that the five rib (release of intracellular bacteria) mutants of Legionella pneumophila are competent for intracellular replication but defective in pore formation-mediated cytolysis and egress from protozoan and mammalian cells. The rib phenotype results from a point mutation (deletion) DeltaG544 in icmT that is predicted to result in the expression of a protein truncated by 32 amino acids from the C-terminus. In contrast to the rib mutants that are capable of intracellular replication, an icmT null mutant was completely defective in intracellular replication within mammalian and protozoan cells, in addition to its defect in pore formation-mediated cytolysis. The icmT wild-type allele complemented the icmT null mutant for both defects of intracellular replication and pore formation-mediated cytolysis and egress from mammalian cells. In contrast, the icmTDeltaG544 allele complemented the icmT null mutant for intracellular growth, but not for the pore-forming activity. Consistent with their defect in pore formation-mediated cytotoxicity in vitro, both mutants failed to cause pulmonary inflammation in A/J mice. Interestingly, the rib mutant was severely defective in intracellular growth within Acanthamoeb...Continue Reading

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Citations

Jan 24, 2004·Microbes and Infection·Maëlle MolmeretYousef Abu Kwaik
Jun 29, 2002·Trends in Microbiology·Maëlle Molmeret, Yousef Abu Kwaik
Apr 9, 2010·Clinical Microbiology Reviews·Hayley J NewtonElizabeth L Hartland
Apr 11, 2007·Infection and Immunity·Marina SanticYousef Abu Kwaik
Feb 15, 2007·Environmental Microbiology·Hubert HilbiSimon Urwyler
May 20, 2004·International Journal of Medical Microbiology : IJMM·Dina M BitarYousef Abu Kwaik
Mar 19, 2014·Frontiers in Cellular and Infection Microbiology·Jianwu PeiThomas A Ficht
Nov 20, 2003·The Journal of Biological Chemistry·Guillaume DuménilRalph R Isberg
Mar 14, 2003·Microbiology·O A Terry AlliYousef Abu-Kwaik
Feb 28, 2004·Science·John ChenHoward A Shuman

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