The C-terminus of neuronal Kv2.1 channels is required for channel localization and targeting but not for NMDA-receptor-mediated regulation of channel function.

Neuroscience
S B Baver, Kristen M S O'Connell

Abstract

The delayed rectifier voltage-gated potassium channel Kv2.1 underlies a majority of the somatic K(+) current in neurons and is particularly important for regulating intrinsic neuronal excitability. Various stimuli alter Kv2.1 channel gating as well as localization of the channel to cell-surface cluster domains. It has been postulated that specific domains within the C-terminus of Kv2.1 are critical for channel gating and sub-cellular localization; however, the distinct regions that govern these processes remain elusive. Here we show that the soluble C-terminal fragment of the closely related channel Kv2.2 displaces Kv2.1 from clusters in both rat hippocampal neurons and HEK293 cells, however neither steady-state activity nor N-methyl-d-aspartate (NMDA)-dependent modulation is altered in spite of this non-clustered localization. Further, we demonstrate that the C-terminus of Kv2.1 is not necessary for steady-state gating, sensitivity to intracellular phosphatase or NMDA-dependent modulation, though this region is required for localization of Kv2.1 to clusters. Thus, the molecular determinants of Kv2.1 localization and modulation are distinct regions of the channel that function independently.

References

Feb 1, 1997·Journal of Neuroscience Methods·G J Brewer
Nov 14, 1997·Molecular Pharmacology·H MurakoshiJ S Trimmer
Jun 15, 2004·Nature Neuroscience·Hiroaki MisonouJames S Trimmer
Jun 29, 2004·Nature Neuroscience·D James Surmeier, Robert Foehring
Apr 28, 2005·Journal of Cell Science·Kristen M S O'Connell, Michael M Tamkun
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mark HowarthAlice Y Ting
Jun 14, 2005·Neurotoxicology·Hiroaki MisonouJames S Trimmer
Dec 2, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hiroaki MisonouJames S Trimmer
Jan 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Durga P Mohapatra, James S Trimmer
Aug 19, 2006·Science·Kang-Sik ParkJames S Trimmer
Sep 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristen M S O'ConnellMichael M Tamkun
Dec 29, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hiroaki MisonouJames S Trimmer
Apr 5, 2007·Nature Protocols·Stefanie Kaech, Gary Banker
Oct 25, 2007·Biochemical Society Transactions·D P MohapatraJ S Trimmer
May 9, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Durga P MohapatraJames S Trimmer
Aug 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Patrick J MulhollandL Judson Chandler
Feb 19, 2009·Journal of Cell Science·Xiao-Qing DaiPatrick E Macdonald
Mar 6, 2010·The Journal of Biological Chemistry·Yoshitaka KihiraHiroaki Misonou
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristen M S O'ConnellMichael M Tamkun
Apr 27, 2011·The Journal of General Physiology·Leigh D PlantSteve A N Goldstein

❮ Previous
Next ❯

Citations

Jan 18, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Philip D FoxMichael M Tamkun
Aug 29, 2020·International Journal of Molecular Sciences·Chung-Yang YehElias Aizenman
Jun 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ben JohnsonMichael M Tamkun
Apr 25, 2015·Journal of Cell Science·Philip D FoxMichael M Tamkun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Kristen M S O'ConnellMichael M Tamkun
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
D P MohapatraJ S Trimmer
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Hiroaki MisonouJ S Trimmer
© 2021 Meta ULC. All rights reserved