The C-Terminus Tail Regulates ERK3 Kinase Activity and Its Ability in Promoting Cancer Cell Migration and Invasion.

International Journal of Molecular Sciences
Lobna ElkhadragyWeiwen Long

Abstract

Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein kinase (MAPK) family. It harbors a kinase domain in the N-terminus and a long C-terminus extension. The C-terminus extension comprises a conserved in ERK3 and ERK4 (C34) region and a unique C-terminus tail, which was shown to be required for the interaction of ERK3 with the cytoskeletal protein septin 7. Recent studies have elucidated the role of ERK3 signaling in promoting the motility and invasiveness of cancer cells. However, little is known about the intramolecular regulation of the enzymatic activity and cellular functions of ERK3. In this study, we investigated the role of the elongated C-terminus extension in regulating ERK3 kinase activity and its ability to promote cancer cell migration and invasion. Our study revealed that the deletion of the C-terminus tail greatly diminishes the ability of ERK3 to promote the migration and invasion of lung cancer cells. We identified two molecular mechanisms underlying this effect. Firstly, the deletion of the C-terminus tail decreases the kinase activity of ERK3 towards substrates, including the oncogenic protein steroid receptor co-activator 3 (SRC-3), an important downstream targ...Continue Reading

References

Apr 12, 1996·The Journal of Biological Chemistry·M ChengM H Cobb
Apr 5, 2001·The Journal of Biological Chemistry·M K AbeM R Rosner
Nov 19, 2004·The Journal of Biological Chemistry·Marcus Buschbeck, Axel Ullrich
Aug 9, 2005·The International Journal of Biochemistry & Cell Biology·Theresa MikalsenUgo Moens
Sep 16, 2006·The Journal of Biological Chemistry·Shashi KantMatthias Gaestel
Dec 13, 2006·Biochimica Et Biophysica Acta·Phillipe Coulombe, Sylvain Meloche
Aug 25, 2009·Nature Reviews. Cancer·Jianming XuBert W O'Malley
Nov 18, 2009·Cancer Investigation·Song XuPeiyu Pu
Dec 26, 2009·Journal of Neuro-oncology·Zhi-fan JiaPei-yu Pu
Feb 15, 2011·The Journal of Biological Chemistry·Alina De la Mota-PeynadoAlexander Beeser
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Marie Cargnello, Philippe P Roux
Feb 9, 2012·Nature Reviews. Molecular Cell Biology·Serge Mostowy, Pascale Cossart
Apr 17, 2012·The Journal of Clinical Investigation·Weiwen LongBert W O'Malley
Jan 30, 2014·PloS One·Miriam MarquisNathalie Labrecque
Mar 4, 2014·Journal of Cellular Physiology·Wei WangWeiwen Long
Dec 19, 2014·Immunology·Julien SiroisNathalie Labrecque
Nov 21, 2015·Cell Adhesion & Migration·Rania Al-MahdiClaire M Wells
Jan 13, 2017·Molecular Oncology·Lobna ElkhadragyWeiwen Long
Jan 14, 2020·Bio-protocol·Lobna Elkhadragy, Weiwen Long

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Citations

Sep 5, 2020·International Journal of Molecular Sciences·Thomas KubinManfred Richter

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