The C53/C37 subcomplex of RNA polymerase III lies near the active site and participates in promoter opening.

The Journal of Biological Chemistry
George A KassavetisEunjung Shim

Abstract

The C53 and C37 subunits of RNA polymerase III (pol III) form a subassembly that is required for efficient termination; pol III lacking this subcomplex displays increased processivity of RNA chain elongation. We show that the C53/C37 subcomplex additionally plays a role in formation of the initiation-ready open promoter complex similar to that of the Brf1 N-terminal zinc ribbon domain. In the absence of C53 and C37, the transcription bubble fails to stably propagate to and beyond the transcriptional start site even when the DNA template is supercoiled. The C53/C37 subcomplex also stimulates the formation of an artificially assembled elongation complex from its component DNA and RNA strands. Protein-RNA and protein-DNA photochemical cross-linking analysis places a segment of C53 close to the RNA 3' end and transcribed DNA strand at the catalytic center of the pol III elongation complex. We discuss the implications of these findings for the mechanism of transcriptional termination by pol III and propose a structural as well as functional correspondence between the C53/C37 subcomplex and the RNA polymerase II initiation factor TFIIF.

References

Mar 1, 1992·Molecular and Cellular Biology·J M NigroB Vogelstein
Jul 5, 1992·Journal of Molecular Biology·G A KassavetisE P Geiduschek
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·G A KassavetisM Pisano
Mar 1, 1995·Molecular and Cellular Biology·S A ShaabanB D Hall
Aug 5, 1994·Science·E NudlerM Kashlev
Jan 28, 1994·Journal of Molecular Biology·H MatsuzakiE P Geiduschek
Feb 1, 1993·Molecular and Cellular Biology·B BartholomewE P Geiduschek
Jan 1, 1995·Methods in Enzymology·B BartholomewE P Geiduschek
Sep 13, 1996·The Journal of Biological Chemistry·W PowellD Reines
Sep 5, 1997·The Journal of Biological Chemistry·E V Bobkova, B D Hall
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·G A KassavetisE P Geiduschek
Aug 14, 1998·Molecular Cell·I SidorenkovM Kashlev
Jun 23, 2001·Journal of Molecular Biology·E P Geiduschek, G A Kassavetis
Oct 17, 2002·Genes & Development·Laura Schramm, Nouria Hernandez
Oct 3, 2003·The Journal of Biological Chemistry·Lynn M ZieglerAlfred S Ponticelli
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Sep 2, 2005·Molecular and Cellular Biology·Finn Werner, Robert O J Weinzierl
Oct 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·George A KassavetisE Peter Geiduschek

❮ Previous
Next ❯

Citations

Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Pavel ČabartDonal S Luse
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chih-Chien WuHung-Ta Chen
Mar 23, 2011·Nucleic Acids Research·Andrea OrioliGiorgio Dieci
Mar 8, 2012·Nucleic Acids Research·Stefan JennebachPatrick Cramer
Sep 24, 2011·Genes & Development·Claudia BlattnerPatrick Cramer
Feb 13, 2013·Molecular and Cellular Biology·Aneeshkumar G Arimbasseri, Richard J Maraia
Oct 16, 2012·Biochimica Et Biophysica Acta·Joël AckerOlivier Lefebvre
Dec 4, 2012·Biochimica Et Biophysica Acta·Magdalena Boguta
Nov 7, 2012·Biochimica Et Biophysica Acta·Giorgio DieciRoberto Ferrari
Oct 27, 2012·Biochimica Et Biophysica Acta·Aneeshkumar G ArimbasseriRichard J Maraia
Nov 21, 2012·Biochimica Et Biophysica Acta·Robyn D Moir, Ian M Willis
Nov 26, 2015·Nature·Niklas A HoffmannChristoph W Müller
Oct 23, 2010·The EMBO Journal·Carlos Fernández-TorneroChristoph W Müller
Jun 30, 2011·Protein Science : a Publication of the Protein Society·Haruhiko EharaShigeyuki Yokoyama
Sep 2, 2011·RNA Biology·Carlos Fernández-TorneroChristoph W Müller
Oct 5, 2010·Cell Cycle·Hélène Dumay-OdelotMartin Teichmann
Mar 1, 2012·Molecular Cell·Alessandro Vannini, Patrick Cramer
Aug 28, 2010·Molecular Cell·Sebastian R GeigerPatrick Cramer
Oct 5, 2010·Cell·Alessandro VanniniPatrick Cramer
Apr 10, 2016·The FEBS Journal·Niklas A HoffmannChristoph W Müller
Mar 16, 2017·Genes & Development·Meghdad YeganehNouria Hernandez
Jan 19, 2018·Nature·Guillermo Abascal-PalaciosAlessandro Vannini
Jan 19, 2018·Nature·Matthias K VorländerChristoph W Müller
Jan 13, 2018·Annual Review of Biochemistry·Ian M Willis, Robyn D Moir
Jun 24, 2014·Molecular Systems Biology·Atsushi FukuohHoward T Jacobs
Aug 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Aravind RamanathanNayef Jarrous
Feb 10, 2021·Nature Structural & Molecular Biology·Mathias GirbigChristoph W Müller
Feb 10, 2021·Nature Structural & Molecular Biology·Qianmin WangJian Wu
Mar 7, 2021·Cell Research·Liang LiYanhui Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.