The Ca2+ -induced pore opening in mitochondria energized by succinate-ferricyanide electron transport

FEBS Letters
Boris F KrasnikovDmitry B Zorov

Abstract

The oxygen dependence of the mitochondria permeability transition pore was under study in non-respiring rat liver mitochondria. Oxygen in the medium was depleted by saturation of the incubation medium with N2 and spontaneously by mitochondrial respiration followed by the addition of glucose/glucose oxidase. After the anaerobic state had been reached, ferricyanide has been added to support succinate-driven energization in the absence of oxygen. In the other set of the experiments KCN was added to block operation of the respiratory chain under aerobic conditions. Again, ferricyanide was added as an electron acceptor. Superoxide dismutase was added to trap superoxide anion radicals. Under either hypoxic conditions or in the presence of cyanide, calcium ions were shown to induce the permeability transition. The concentration of Ca2+ required was lower than under conditions of active respiration. In both cases, the transition was prevented by cyclosporine A.

References

Jul 1, 1979·Archives of Biochemistry and Biophysics·R A Haworth, D R Hunter
Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Jan 17, 1985·The New England Journal of Medicine·J M McCord
Aug 15, 1988·The Biochemical Journal·J B Hoek, J Rydström
Aug 18, 1988·Biochimica Et Biophysica Acta·D Carbonera, G F Azzone
Dec 1, 1993·Journal of Molecular and Cellular Cardiology·E J Griffiths, A P Halestrap
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Sep 1, 1996·The Journal of Experimental Medicine·P MarchettiG Kroemer
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·P Bernardi, V Petronilli
May 9, 1997·The Journal of Biological Chemistry·L ScorranoP Bernardi
Jun 1, 1997·Archives of Biochemistry and Biophysics·T GudzS Novgorodov
Jun 1, 1997·Bioscience Reports·V P Skulachev

❮ Previous
Next ❯

Citations

Jan 5, 2008·Stroke; a Journal of Cerebral Circulation·Wen-hua ZhangRobert M Friedlander
Nov 5, 2014·Biochemistry. Biokhimii︠a︡·T A TrendelevaR A Zvyagilskaya
Nov 13, 2001·The Journal of Biological Chemistry·Silvio ArpagausRoland Braendle
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·D B ZorovE Y Plotnikov
Apr 17, 1998·Biophysical Journal·J HüserL A Blatter
Jun 24, 2009·Biochimica Et Biophysica Acta·Egor Y PlotnikovDmitry B Zorov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.