The Ca2+ homeostasis defects in a pgm2Delta strain of Saccharomyces cerevisiae are caused by excessive vacuolar Ca2+ uptake mediated by the Ca2+-ATPase Pmc1p

The Journal of Biological Chemistry
D P AielloD M Bedwell

Abstract

Loss of the major isoform of phosphoglucomutase (PGM) causes an accumulation of glucose 1-phosphate when yeast cells are grown with galactose as the carbon and energy source. Remarkably, the pgm2Delta strain also exhibits a severe imbalance in intracellular Ca(2+) homeostasis when grown under these conditions. In the present study, we examined how the pgm2Delta mutation alters yeast Ca(2+) homeostasis in greater detail. We found that a shift from glucose to galactose as the carbon source resulted in a 2-fold increase in the rate of cellular Ca(2+) uptake in wild-type cells, whereas Ca(2+) uptake increased 8-fold in the pgm2Delta mutant. Disruption of the PMC1 gene, which encodes the vacuolar Ca(2+)-ATPase Pmc1p, suppressed the Ca(2+)-related phenotypes observed in the pgm2Delta strain. This suggests that excessive vacuolar Ca(2+) uptake is tightly coupled to these defects in Ca(2+) homeostasis. An in vitro assay designed to measure Ca(2+) sequestration into intracellular compartments confirmed that the pgm2Delta mutant contained a higher level of Pmc1p-dependent Ca(2+) transport activity than the wild-type strain. We found that this increased rate of vacuolar Ca(2+) uptake also coincided with a large induction of the unfolded p...Continue Reading

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Citations

Jan 22, 2013·Folia Microbiologica·Seo Young BangSung Goo Park
May 5, 2010·Fungal Genetics and Biology : FG & B·Helen FindonHerbert N Arst
Apr 4, 2006·Biochemical and Biophysical Research Communications·Maria José M TrópiaRogelio L Brandão
Feb 11, 2005·American Journal of Physiology. Cell Physiology·Péter CsutoraAttila Miseta
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May 15, 2021·Microbiological Research·Wanqian LiYuanying Jiang
Mar 8, 2011·Cell Calcium·Kyle W Cunningham

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