PMID: 6412753Oct 12, 1983Paper

The Ca2+ permeability of sarcoplasmic reticulum vesicles. I. Ca2+ outflow in the non-energized state of the calcium pump

Biochimica Et Biophysica Acta
U GerdesJesper V Møller

Abstract

Passive Ca2+ permeability of sarcoplasmic reticulum vesicles has been studied after maximal loading with Ca2+ (150-200 nmol/mg protein) in the presence of Ca2+, MgATP and an ATP generating system of limited capacity. Outflow of accumulated Ca2+ in the non-energized state of the system was studied by depletion of the medium of one of the substrates, either MgATP (by complete consumption) or Ca2+ (by complexation with EGTA). It was found that Ca2+ outflow under these conditions is relatively slow and independent of the medium concentration of Ca2+ (5 X 10(-9)-5 X 10(-5) M) or MgATP (0.7-730 microM). Outflow curves were steep at the beginning of the outflow phase (30-60 nmol/min per mg protein), and outflow proceeded at a much lower rate below 100 nmol Ca2+/mg protein. Outflow could be completely inhibited by La3+. The Ca2+ release curves are not compatible with simple diffusion, and cannot be accounted for by Ca2+ binding inside the vesicles. Neither are our observations consistent with permeation mediated via the Ca2+ translocation sites involved in active transport. We suggest that non-energized Ca2+ outflow may proceed by a process of ion-exchange through negatively charged, water-filled channels in the membrane, the propertie...Continue Reading

References

Apr 21, 1975·Biochimica Et Biophysica Acta·G Meissner
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Apr 10, 1978·Biochimica Et Biophysica Acta·W Hasselbach
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Jan 30, 1971·FEBS Letters·M Makinose, W Hasselbach

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Citations

Sep 28, 2000·Biochemical and Biophysical Research Communications·L de Meis
Apr 5, 2000·The Journal of Biological Chemistry·P ChampeilM le Maire
Oct 1, 1995·Bioscience Reports·H Wolosker, L de Meis
Feb 28, 1985·Biochimica Et Biophysica Acta·J J Feher, G B Lipford
Sep 10, 1985·Biochimica Et Biophysica Acta·J J Feher, G B Lipford

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