The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility

Molecular Biology of the Cell
Hans P KienerMichael B Brenner

Abstract

Cadherins mediate homophilic cell adhesion and contribute to tissue morphogenesis and architecture. Cadherin cell adhesion contacts are actively remodeled and impact cell movement and migration over other cells. We found that expression of a mutant cadherin-11 lacking the cytoplasmic juxtamembrane domain (JMD) diminished the turnover of alpha-catenin at adherens junctions as measured by fluorescence recovery after photobleaching. This resulted in markedly diminished cell intercalation into monolayers reflecting reduced cadherin-11-dependent cell motility on other cells. Furthermore, the actin cytoskeleton in cadherin-11 deltaJMD cells revealed a more extensive cortical F-actin ring that correlated with significantly higher levels of activated Rac1. Together, these data implicate the cadherin-11 cytoplasmic JMD as a regulator of alpha-catenin turnover at adherens junctions and actin-cytoskeletal organization that is critical for intercellular motility and rearrangement in multicellular clusters.

References

Feb 1, 1992·The Journal of Cell Biology·M Ozawa, R Kemler
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K HerrenknechtR Kemler
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·T S JouJ A Marrs
Jul 1, 1994·The Journal of Cell Biology·W M Brieher, B M Gumbiner
Dec 31, 1997·The Journal of Cell Biology·K TakaishiY Takai
Feb 14, 1998·The Journal of Cell Biology·J M HigginsM B Brenner
Mar 31, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S GetsiosC D MacCalman
Feb 11, 1999·The Journal of Cell Biology·P NiewiadomskaU Tepass
Mar 24, 1999·The Journal of Cell Biology·C D Nobes, A Hall
May 4, 1999·The Journal of Cell Biology·S AonoM Takeichi
Jan 12, 2000·The Journal of Cell Biology·M A ThoresonA B Reynolds
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L BakiN K Robakis
Jul 13, 2001·Current Opinion in Genetics & Development·A Locascio, M A Nieto
Jul 18, 2001·The Journal of Biological Chemistry·N K NorenK Burridge
Feb 12, 2002·Nature Cell Biology·Yasuyuki FujitaWalter Birchmeier
Sep 6, 2002·The Journal of Cell Biology·Sophie CharrasseCécile Gauthier-Rouvière
Nov 13, 2002·The Journal of Cell Biology·Renee C IretonAlbert B Reynolds
Mar 1, 2003·The Journal of Biological Chemistry·Felipe Palacios, Crislyn D'Souza-Schorey
Nov 12, 2003·The Journal of Cell Biology·Michael A DavisAlbert B Reynolds
Dec 3, 2003·Nature Cell Biology·Agnieszka KobielakElaine Fuchs
Mar 6, 2004·Science·W James Nelson, Roel Nusse
Dec 22, 2004·The Journal of Experimental Medicine·Xavier ValenciaMichael B Brenner
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Barry M Gumbiner
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson

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Citations

Aug 15, 2009·Cell and Tissue Research·Werner W FrankeSebastian Pieperhoff
Jul 18, 2008·Molecular Biology of the Cell·Alexi KissSergey M Troyanovsky
Jan 11, 2014·PloS One·Davina TondeleirChristophe Ampe
Apr 11, 2014·Modern Rheumatology·Hideyuki ShibuyaShuichi Matsuda
Sep 20, 2006·The Journal of Cell Biology·Masahiro Yanagisawa, Panos Z Anastasiadis
May 5, 2012·Trends in Cell Biology·Julia BraschLawrence Shapiro
Jan 24, 2012·Best Practice & Research. Clinical Rheumatology·Hans P Kiener, Thomas Karonitsch
Oct 20, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Honglei LiCunshuan Xu
May 1, 2009·Arthritis and Rheumatism·Hans P KienerMichael B Brenner
Sep 30, 2008·Arthritis and Rheumatism·Bernard VandoorenDominique Baeten
Mar 27, 2010·International Journal of Cancer. Journal International Du Cancer·Marilena VeredTuula Salo
Mar 3, 2010·Immunological Reviews·Sook Kyung ChangMichael B Brenner
Aug 15, 2006·Current Opinion in Cell Biology·René-Marc MègeMireille Lambert
Jun 25, 2008·Cytotherapy·A D HoW Franke
Apr 9, 2015·Frontiers in Neuroanatomy·Nora-Emöke SzabóGonzalo Alvarez-Bolado
Apr 2, 2013·Biochimica Et Biophysica Acta·M GeletuL Raptis
Jun 18, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Derek C SungJonathan T Butcher
Dec 19, 2014·Bulletin of Mathematical Biology·B Roy Frieden, Robert A Gatenby
Dec 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angelica OrtizSue-Hwa Lin
Nov 8, 2017·Journal of Proteome Research·Yu-Chen LeeSue-Hwa Lin

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