The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Takafumi KonishiAsako Sugimoto

Abstract

DEAD/H-box proteins are involved in various aspects of RNA metabolism. Here we report the developmental function of a DEAD-box protein, DDX-23, in Caenorhabditis elegans, which has significant homology with the yeast splicing factor PRP28. We found by RNAi and mutant analyses that DDX-23 is essential for both embryonic and post-embryonic development, and required for differentiation of the majority of somatic tissues. When the germline function of ddx-23 was inhibited, hermaphrodite animals showed a reduced number of germ cells and failed to switch from spermatogenesis to oogenesis. These phenotypes were similar to those of the mutants of the three DEAH-box proteins (MOG-1, MOG-4, and MOG-5) whose yeast orthologs are involved in the pre-mRNA splicing pathway. We speculate that DDX-23 functions with the three MOG proteins in the same pathway to regulate tissue differentiation, robust germline proliferation, and the sperm/oocyte switch through modulations of ribonucleoprotein complexes.

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Citations

Feb 9, 2010·Genome Génome / Conseil National De Recherches Canada·Hilary Racher, Dave Hansen
Jun 19, 2009·BMC Developmental Biology·Ryuji MinasakiAdrian Streit
Oct 15, 2011·Parasitology International·Renu Tuteja
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chris WangDave Hansen
Apr 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jessica Amrozowicz KerinsTim Schedl
Jun 19, 2016·G3 : Genes - Genomes - Genetics·Megan P WoodJennifer A Schisa
Mar 14, 2017·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Changxu TianZhanjiang Liu

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