The Caenorhabditis elegans rsd-2 and rsd-6 genes are required for chromosome functions during exposure to unfavorable environments.

Genetics
Wang HanLisa Timmons

Abstract

In Caenorhabditis elegans, exogenous dsRNA can elicit systemic RNAi, a process that requires the function of many genes. Considering that the activities of many of these genes are also required for normal development, it is surprising that exposure to high concentrations of dsRNA does not elicit adverse consequences to animals. Here, we report inducible phenotypes in attenuated C. elegans strains reared in environments that include nonspecific dsRNA and elevated temperature. Under these conditions, chromosome integrity is compromised in RNAi-defective strains harboring mutations in rsd-2 or rsd-6. Specifically, rsd-2 mutants display defects in transposon silencing, while meiotic chromosome disjunction is affected in rsd-6 mutants. RSD-2 proteins localize to multiple cellular compartments, including the nucleolus and cytoplasmic compartments that, in part, are congruent with calreticulin and HAF-6. We considered that the RNAi defects in rsd-2 mutants might have relevance to membrane-associated functions; however, endomembrane compartmentalization and endocytosis/exocytosis markers in rsd-2 and rsd-6 mutants appear normal. The mutants also possess environmentally sensitive defects in cell-autonomous RNAi elicited from transgene-d...Continue Reading

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Citations

Sep 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aisa SakaguchiShawn Ahmed
Aug 4, 2012·RNA Biology·Chi Zhang, Gary Ruvkun
May 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Luciana E LeopoldShawn Ahmed
Mar 17, 2010·The International Journal of Biochemistry & Cell Biology·Sylvia E J Fischer
May 14, 2009·Journal of Neuroscience Methods·Toshifumi TakahashiMakoto Mizunami
Jul 4, 2015·Genesis : the Journal of Genetics and Development·Antony M Jose
Sep 18, 2012·Current Biology : CB·Andrea HinasCraig P Hunter
Jan 23, 2015·EMBO Reports·Anikó I NagyHoward A Baylis

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