The calcium action potential and a prolonged calcium dependent after-hyperpolarization in mouse neuroblastoma cells

The Journal of Physiology
W H Moolenaar, I Spector

Abstract

1. Action potentials elicited in solutions with elevated [Ca2+] (1.8-40 mM) have been studied in differentiated cells of mouse neuroblastoma clone N1E-115 in tissue culture. 2. The action potential in high [Ca2+] solutions containing eithr Na+ or Tris is followed by a prolonged after-hyperpolarization (a.h.p.) lasting 0.5-4 sec. The a.h.p. reverses sign between -75 and -85 mV. 3. Externally applied tetraethylammonium (TEA, 15 mM) increases the Ca2+ spike overshoot, prolongs the falling phase and enhances the a.h.p. duration. The a.h.p. is inhibited by Ca2+ antagonists such as La3+, Co2+ and Mn2+. 4. After replacement of Ca2+ by Ba+ or Sr2+ (20mM) action potentials can still be elicited in Na+-free solution, but no a.h.p. is observed. 5. Increasing [Ca2+] from 1.8 up to 20 mM results in an increased capability of neuroblastoma cells to fire repetitively and in a consistent reduction of the firing rate from about 4-10 sec-1 to 0.5-1.8 sec-1. 6. It is concluded that Ca2+ entry during the action potential activates a TEA-resistant K+ conductance which gives rise to the prolonged a.h.p. Data from repetitively firing cells are consistent with the view that the a.h.p. plays a role in the regulation of low-frequency firing.

Citations

Apr 1, 1984·Journal of Neurochemistry·C PoustisJ P Vincent
Mar 1, 1983·Journal of Neurochemistry·E El-Fakahany, E Richelson
Aug 26, 2014·Biological Psychiatry·Michel EngelnPierre-Olivier Fernagut
Jan 1, 1984·Annals of Neurology·T Narahashi
Jun 1, 1989·Pflügers Archiv : European journal of physiology·K Morita, Y Katayama
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Aug 15, 1988·FEBS Letters·F GilbertM C Glick
Sep 13, 2017·Soft Matter·M I Pérez-Camacho, J C Ruiz-Suárez
Jul 1, 1981·The American Journal of Physiology·B Ribalet, P M Beigelman
Mar 26, 1984·Life Sciences·R J Miller, S B Freedman

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