The calcium channel beta2 (CACNB2) subunit repertoire in teleosts.

BMC Molecular Biology
Alicia M EbertDeborah M Garrity

Abstract

Cardiomyocyte contraction is initiated by influx of extracellular calcium through voltage-gated calcium channels. These oligomeric channels utilize auxiliary beta subunits to chaperone the pore-forming alpha subunit to the plasma membrane, and to modulate channel electrophysiology 1. Several beta subunit family members are detected by RT-PCR in the embryonic heart. Null mutations in mouse beta2, but not in the other three beta family members, are embryonic lethal at E10.5 due to defects in cardiac contractility 2. However, a drawback of the mouse model is that embryonic heart rhythm is difficult to study in live embryos due to their intra-uterine development. Moreover, phenotypes may be obscured by secondary effects of hypoxia. As a first step towards developing a model for contributions of beta subunits to the onset of embryonic heart rhythm, we characterized the structure and expression of beta2 subunits in zebrafish and other teleosts. Cloning of two zebrafish beta2 subunit genes (beta2.1 and beta2.2) indicated they are membrane-associated guanylate kinase (MAGUK)-family genes. Zebrafish beta2 genes show high conservation with mammals within the SH3 and guanylate kinase domains that comprise the "core" of MAGUK proteins, but...Continue Reading

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Citations

Oct 2, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brittany B LewisMargaret S Saha
Oct 19, 2016·Scientific Reports·He WangJianping Bin
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yelena ChernyavskayaDeborah M Garrity
Dec 4, 2014·The Journal of Biological Chemistry·Amy LeeFrançoise Haeseleer

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Datasets Mentioned

BETA
AAH76523.1
EU301442
EU301434
BK006349
AAA75519.1
ABU93250.1
ABU93253.1

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

BLASTP
ClustalW
PAUP
BLAST
BLAT
MEGABLAST
TBLASTN
BLAT Browser Browser
TreePuzzle
Ensemble

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