The calcium current and the activation of a slow potassium conductance in voltage-clamped mouse neuroblastoma cells

The Journal of Physiology
W H Moolenaar, I Spector

Abstract

1. The Ca2+ inward current (ICa) and a slow outward current in differentiated cells of mouse neuroblastoma clone N1E-115 have been studied under voltage-clamp conditions. 2. ICa shows voltage- and time-dependent inactivation when evoked by step-wise depolarizations in Na+-free solution containing high [Ca2+] (20 nM) and tetraethylammonium (TEA, 25 mM). Ba2+ and Sr2+ can substitute for Ca2+. 3. Holding potentials below -70 mV maximal activate ICa. Half inactivation occurs at -56 mV and ICa is completely inactivated beyond holding levels of -30 mV. Maximum peak currents are of the order of 10(-4) A/cm2 and the reversal potential ranges from +40 to +60 mV. The ICa inactivation time course follows first-order kinetics with a voltage-depedent time constant ranging from 25 to 100 msec. 4. The striking resemblance between ICa and the Ca2+ current in the unfertilized mouse oocyte (Okamoto, Takahashi & Yamashita, 1977) is discussed. 5. A slow outward current with a rise time of several seconds is recorded on voltage steps beyond -20 mV in high [Ca2+] solutions. It is carried primarily by K+ on account of the value of the reversal potential and its dependence on [K]0. This K+ current is TEA-insensitive and is blocked by Ca2+ antagonists....Continue Reading

Citations

Jan 1, 1984·Neir̆ofiziologiia = Neurophysiology·N S VeselovskiĭA F Fomina
Jan 1, 1983·The Journal of Membrane Biology·M Kameyama
Jun 19, 2004·The International Journal of Neuroscience·Waleed B Alshuaib, Mini V Mathew
Jun 19, 2004·The International Journal of Neuroscience·Senol DaneAysenur Aksoy
May 1, 1986·Acta Physiologica Scandinavica·B HedlundA Sydbom
Mar 1, 1983·Journal of Neurochemistry·E El-Fakahany, E Richelson
Nov 19, 1986·Brain Research·K TakedaY Berwald-Netter
Sep 30, 1999·Microscopy Research and Technique·G R Van Den BrinkM P Peppelenbosch
Apr 1, 1985·Pflügers Archiv : European journal of physiology·J L BossuJ M Thomann
Jan 1, 1984·Annals of Neurology·T Narahashi
Jul 1, 1985·Pflügers Archiv : European journal of physiology·M GalvanP Franz
Jul 1, 1984·Pflügers Archiv : European journal of physiology·H P VijverbergM Lazdunski
Apr 1, 1985·Pflügers Archiv : European journal of physiology·J W Deitmer, R Eckert
Nov 1, 1981·Biochemical Pharmacology·E Richelson, E El-Fakahany
Nov 1, 1984·Biochimie·M Hugues
Mar 21, 1983·Biochimica Et Biophysica Acta·R D Moore
Apr 14, 1989·Biochimica Et Biophysica Acta·A Majander, M Wikström

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.