The calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function

The Plant Journal : for Cell and Molecular Biology
Andrew G McCubbinSimon Gilroy

Abstract

In the aleurone cells of the cereal grain, gibberellic acid (GA) induces the secretion of hydrolases that mobilize endosperm reserves to fuel early seedling growth. GA is known to trigger a range of cellular responses, including increases in cytoplasmic calcium, vacuolar reserve mobilization, gene transcription, and the synthesis and secretion of hydrolases. To further define elements of the Ca2+-dependent GA response machinery, we have cloned a Ca2+-dependent protein kinase (HvCDPK1) from these cells. Although expression of an inactivated (D140N) version of this kinase did not affect GA-induced gene expression or changes in cytosolic Ca2+, it did inhibit secretion, cell vacuolation, and vacuolar acidification, all responses linked to the GA response. Additionally, recombinant wild-type HvCDPK1 activated the V-type H(+)-ATPase present in isolated aleurone vacuoles. These results suggest HvCDPK1 may mediate Ca2+-dependent events of the GA response, such as control of vacuolar function, that lie downstream of transcriptional regulation.

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Gilroy, R L Jones
Jan 1, 1991·International Review of Cytology·R L Jones, J V Jacobsen
Oct 1, 1989·The Plant Cell·W R MarcotteR S Quatrano
Jun 1, 1995·Plant Physiology·M Abo-el-Saad, R Wu
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Gómez-CadenasM K Walker-Simmons
Mar 9, 2000·Trends in Plant Science·A Lovegrove, R Hooley
Mar 31, 2000·Trends in Plant Science·A C HarmonJ F Harper
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·I HwangJ F Harper
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·Y SaijoK Izui
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·U Homann, M Tester
Apr 16, 2002·Trends in Plant Science·Heven SzeLincoln Taiz
Jun 1, 1996·Plant Physiology·R. C. SchuurinkR. L. Jones
Dec 1, 1996·The Plant Cell·S. J. Swanson, R. L. Jones

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Citations

Jun 21, 2005·Biochimica Et Biophysica Acta·Mei ZhangYing-Tang Lu
Oct 2, 2009·Plant Physiology·Gregory L RichterSimon Gilroy
Aug 2, 2013·Plant Cell Reports·Chun-Hai Dong, Yan Hong
Oct 9, 2007·Molecular Plant-microbe Interactions : MPMI·Gerald FreymarkRalph Panstruga
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Jeffrey F Harper, Alice Harmon
Feb 18, 2009·Plant Molecular Biology·Pablo Rubén GargantiniRita María Ulloa
Jan 24, 2006·FEBS Letters·Anne Hong-HermesdorfKarin Schumacher
May 10, 2012·Critical Reviews in Biotechnology·Maryam SarwatXiangyang Hu
Jan 5, 2011·Nitric Oxide : Biology and Chemistry·Hirofumi YoshiokaShuta Asai
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Ignacio Rubio-SomozaPilar Carbonero
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Miguel Angel Moreno-RisuenoPilar Carbonero
Dec 17, 2009·The Biochemical Journal·Thomas A DeFalcoWayne A Snedden
Mar 18, 2008·Journal of Plant Physiology·Katarzyna Kabała, Grazyna Kłobus
Apr 12, 2005·Plant Molecular Biology·Hirosato KonishiSetsuko Komatsu
Jul 20, 2005·Plant Molecular Biology·Md Monowar Karim KhanSetsuko Komatsu
Oct 11, 2005·Journal of Proteome Research·Hirosato KonishiSetsuko Komatsu
Aug 11, 2010·Journal of Plant Physiology·Katarzyna KabałaGrazyna Kłobus
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Katarzyna Kabała, Małgorzata Janicka-Russak

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