PMID: 2106509Mar 1, 1990Paper

The CAM-OCT plasmid enhances UV responses of Pseudomonas aeruginosa recA mutants

Journal of Bacteriology
D L McBeth

Abstract

The effect of the CAM-OCT plasmid on responses to UV irradiation of Pseudomonas aeruginosa recA mutants was characterized. Mutant alleles examined included rec-1, rec-2, and recA7::Tn501. The plasmid substantially enhanced both survival and mutagenesis of RecA- cells after treatment with UV light. Survival of the RecA-(CAM-OCT) cells after UV irradiation was intermediate between that seen in the wild-type P. aeruginosa PAO1 and the increased survival seen in PAO1(CAM-OCT) cells. Mutability was quantitated by the reversion to carbenicillin resistance of strains carrying a bla(Am) mutation on a derivative of plasmid RP1. UV-induced mutagenesis of CAM-OCT carrying recA mutants occurred at levels comparable to that seen in PAO1(CAM-OCT). The ability of CAM-OCT plasmid to suppress the recombination deficiency in recA mutants was tested by assaying for bacteriophage F116L-generalized transduction of a Tn7 insertion in the alkane utilization genes of CAM-OCT. Transduction of the Tn7 insertion was not detected in RecA-(CAM-OCT) strains but was easily seen in PAO1(CAM-OCT), indicating that the plasmid does not encode a recA analog. The results indicate that the CAM-OCT UV response genes are expressed in RecA- cells, which differs from r...Continue Reading

References

Jan 1, 1977·Journal of General Microbiology·P LehrbachG A Jacoby
Sep 1, 1979·Journal of Bacteriology·M FennewaldJ Shapiro
Aug 1, 1975·Journal of Bacteriology·A GrundM Toepfer
Jul 1, 1987·Molecular & General Genetics : MGG·Y Sano, M Kageyama
Aug 1, 1985·Journal of Bacteriology·T A Kokjohn, R V Miller
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H ShinagawaA Nakata
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S E BurckhardtH Echols
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T NohmiG C Walker
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S G Sedgwick, P A Goodwin
Apr 1, 1974·Mutation Research·P M Chandler, V Krishnapillai
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A M Chakrabarty
Jan 1, 1982·Molecular & General Genetics : MGG·M Caruso, J A Shapiro
Jan 1, 1984·Molecular & General Genetics : MGG·D J OwenJ A Shapiro
Jan 1, 1983·Molecular & General Genetics : MGG·R FrühD Haas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cavernous Hemangioma

Cavernous hemangioma is a blood vessel defect or benign tumor that leads to leakage of blood to the surrounding tissues. This can occur in several organs including the brain, which can lead to seizures. Discover the latest research on cavernous hemangiomas here.