The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements.

The Journal of Biological Chemistry
L L HeckertJ H Nilson

Abstract

The alpha subunit gene encodes a common subunit shared by all glycoprotein hormones. This single copy gene is expressed in pituitary gonadotropes and thyrotropes of all mammals and in placental trophoblasts of primates and horses. Tandem cAMP response elements (CREs) in the promoter of the human gene are key mediators of this pattern of cell-specific expression. Replacing the palindromic CREs with non-primate variant CREs significantly attenuated activity in trophoblasts but not in gonadotropes. Furthermore, proteins binding the palindromic CRE cross-reacted with antibodies for CREB, CREM, ATF1, ATF2, and c-Jun, while proteins binding the variant CRE cross-reacted only with ATF2 and c-Jun antibodies. The data suggest that ATF2 and c-Jun can activate transcription through the CREs in gonadotropes but not in trophoblasts. Additional analyses indicated that while promoters with either palindromic or variant CREs have similar overall activity in gonadotropes, the variant CREs make a much smaller contribution to promoter activity than their palindromic counterparts. The weaker contribution of the variant CREs is compensated by the activity of two upstream elements present in the promoter. This compensation probably occurs through an...Continue Reading

References

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Citations

Jun 29, 2010·Nature Neuroscience·Kurt W MarekNicholas C Spitzer
Mar 27, 2012·Molecular Endocrinology·April K BinderJohn H Nilson
May 16, 2009·The Journal of Reproduction and Development·Takao SusaYukio Kato
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephen J Winters, Joseph P Moore
Aug 22, 2015·Journal of Molecular Endocrinology·Daniil V PopovOlga L Vinogradova
Dec 31, 1997·The Journal of Biological Chemistry·S BiM Reitman
Aug 19, 2008·Frontiers in Neuroendocrinology·Zvi Naor
Oct 29, 2009·Blood Cells, Molecules & Diseases·Sirisha KodeboyinaBetty S Pace
Nov 3, 2010·Molecular and Cellular Endocrinology·M DelidakiD K Grammatopoulos

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