The Campylobacter jejuni transcriptional regulator Cj1556 plays a role in the oxidative and aerobic stress response and is important for bacterial survival in vivo

Journal of Bacteriology
Ozan GundogduNick Dorrell

Abstract

Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative and aerobic (O(2)) stress. Reannotation of the C. jejuni NCTC11168 genome sequence identified Cj1556 (originally annotated as a hypothetical protein) as a MarR family transcriptional regulator, and further analysis indicated a potential role in regulating the oxidative stress response. A C. jejuni 11168H Cj1556 mutant exhibited increased sensitivity to oxidative and aerobic stress, decreased ability for intracellular survival in Caco-2 human intestinal epithelial cells and J774A.1 mouse macrophages, and a reduction in virulence in the Galleria mellonella infection model. Microarray analysis of gene expression changes in the Cj1556 mutant indicated negative autoregulation of Cj1556 expression and downregulation of genes associated with oxidative and aerobic stress responses, such as katA, perR, and hspR. Electrophoretic mobility shift assays confirmed the binding of recombinant Cj1556 to the promoter region upstream of the Cj1556 gene. cprS, whic...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Jan 1, 1987·Annual Review of Microbiology·H G Boman, D Hultmark
Jan 1, 1995·Annual Review of Entomology·M R Strand, L L Pech
Jan 1, 1995·Annual Review of Microbiology·J W CostertonHilary M Lappin-Scott
Feb 1, 1996·Current Opinion in Immunology·T Muta, S Iwanaga
Jan 1, 1997·Microbiology·J M Ketley
Dec 24, 1997·Medical Microbiology and Immunology·T M WassenaarA Lastovica
May 22, 1998·FEMS Microbiology Letters·Marc M S M WöstenB A van der Zeijst
Oct 10, 1998·Journal of Bacteriology·Arnoud H M van VlietJ M Ketley
Oct 21, 1999·Biochemical and Biophysical Research Communications·I A SlepnevaV V Khramtsov
Feb 19, 2000·Current Opinion in Immunology·C BogdanA Diefenbach
Feb 19, 2000·Current Opinion in Microbiology·J L Imler, J A Hoffmann
Jul 6, 2000·Applied and Environmental Microbiology·D Stead, S F Park
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D J PhilpottP J Sansonetti
Oct 18, 2000·Infection and Immunity·W A DayL A Joens
Mar 27, 2001·FEMS Microbiology Letters·Arnoud H M van VlietJ M Ketley
Mar 27, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O SchmidtM Strand
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B M Allos
Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Sep 21, 2004·Nature Reviews. Microbiology·Ferric C Fang
Sep 28, 2004·The Journal of Biological Chemistry·Steven P Wilkinson, Anne Grove
Mar 9, 2005·Molecular Microbiology·Dennis LintonBrendan W Wren
Jun 7, 2005·Journal of Pharmacological Sciences·Mohammad Hasan ZakiTakaaki Akaike
Aug 17, 2005·FEMS Microbiology Reviews·Máire BegleyColin Hill
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olivia L ChampionBrendan W Wren
Jan 5, 2006·Applied and Environmental Microbiology·Hong T T NguyenChristopher A Miles
Jan 27, 2006·Microbiology·G W P JoshuaBrendan W Wren
Jun 20, 2006·International Journal of Food Microbiology·Anja KlancnikSonja Smole Mozina
May 22, 2007·Microbial Pathogenesis·Roberta Rubesa MihaljevicMaja Abram
Jul 7, 2007·Genome Biology·Jodi R ParrishRussell L Finley
Jan 5, 2008·Infection and Immunity·Nicole M IovineYvette Weinrauch
Jan 15, 2008·Applied and Environmental Microbiology·Rebecca A WeingartenJonathan W Olson
Jan 29, 2008·Journal of Bacteriology·Preeti Malik-KaleMichael E Konkel
Jan 30, 2008·PLoS Pathogens·Robert O Watson, Jorge E Galán
Mar 4, 2008·Journal of Bacteriology·Joshua A Fields, Stuart A Thompson
Dec 9, 2008·International Journal of Food Microbiology·Maja Sikić PogacarSonja Smole Mozina
Jan 14, 2009·Infection and Immunity·Lorna M FriisDiane E Taylor
Apr 23, 2009·Microbiology·Olivia L ChampionRichard W Titball
Feb 2, 2010·The Journal of Infectious Diseases·Olivia L ChampionRichard W Titball
Feb 9, 2010·Applied and Environmental Microbiology·Mark ReuterArnoud H M van Vliet
Apr 28, 2010·Applied Nursing Research : ANR·Jenny B Hamner, Barbara Wilder

Citations

Dec 14, 2011·Clinical and Vaccine Immunology : CVI·Lene N NielsenKaren A Krogfelt
Jan 17, 2016·International Journal of Food Microbiology·Leonard KoolmanDeclan Bolton
Nov 22, 2014·Microbiology·Monalessa F PereiraDenise Ms Bazzolli
Mar 6, 2015·Frontiers in Microbiology·Jong-Chul KimByeonghwa Jeon
Jun 4, 2014·FEMS Microbiology Letters·Christina BronowskiCraig Winstanley
Oct 27, 2017·International Journal of Epidemiology·Roy A SandersonStephen P Rushton
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos

Related Concepts

Bacterial Proteins
Tadpole
Macrophage
Antheraea
Dioxygen
Gene Expression Regulation, Bacterial
Campylobacter jejuni
Oxidative Stress
Microbial Biofilms
Campylobacter jejuni

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.