The Candida albicans Rgd1 is a RhoGAP protein involved in the control of filamentous growth

Fungal Genetics and Biology : FG & B
Frédérique NessDidier Thoraval

Abstract

Rho proteins are essential regulators of polarized growth in eukaryotic cells. These proteins are down-regulated in vivo by specific Rho GTPase Activating Proteins (RhoGAP). We investigated the role of Rgd1 RhoGAP, encoded by the Candida albicans RGD1 gene. We demonstrated that CaCdc42, CaRho3 and CaRho4 proteins had an intrinsic GTPase activity and that CaRgd1 stimulates in vitro GTP hydrolysis of these GTPases. Deletion of RGD1 in C. albicans results in sensitivity to low pH as already described for rgd1Δ in Saccharomyces cerevisiae. The role of Rgd1 in survival at low pH is conserved in the two yeast species as the CaRGD1 gene complements the Scrgd1Δ sensitivity. By tagging the RhoGAP with GFP, we found that CaRgd1 is localized at the tip and cortex of growing cells and during cytokinesis at the septation sites in yeast and filamentous forms. We investigated the effect of CaRgd1 on the control of the polarized growth. Removing CaRGD1 alleles increased filamentous growth and cells lacking CaRgd1 presented longer germ tubes. Conversely, RGD1 overexpression restricted hyphae growth. Our results demonstrate that Rgd1 is critical for filamentous formation in C. albicans especially for filamentous elongation.

References

Jan 1, 1991·Methods in Enzymology·F Sherman
Jan 1, 1988·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·F C OddsP D Simmons
May 14, 1998·Microbiology·Gregor D GilfillanNeil A R Gow
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·D I Johnson
Sep 7, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M B EdmondR P Wenzel
Aug 5, 2000·Fungal Genetics and Biology : FG & B·S H Denison
Sep 27, 2000·Journal of Bacteriology·B EnloeA P Mitchell
Jan 12, 2001·FEMS Microbiology Reviews·J M Gancedo
Aug 6, 2002·Current Opinion in Microbiology·Neil A R GowFrank C Odds
Nov 29, 2002·Eukaryotic Cell·Sophia C UshinskyEkkehard Leberer
Dec 3, 2002·Nature Reviews. Genetics·Judith Berman, Peter E Sudbery
Apr 19, 2003·FEMS Yeast Research·Susan E SmithVivian Berlin
Aug 13, 2003·Eukaryotic Cell·Elisabetta SpreghiniAaron P Mitchell
Jan 7, 2004·Journal of Microbiological Methods·Jacob M HornbyKenneth W Nickerson
Jun 30, 2004·Trends in Microbiology·Peter SudberyJudith Berman
Mar 10, 2005·Eukaryotic Cell·Martine BassilanaRobert A Arkowitz
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carol A Kumamoto
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·K Scheffzek, M R Ahmadian
Feb 10, 2006·Eukaryotic Cell·Martine Bassilana, Robert A Arkowitz
Oct 24, 2006·Current Opinion in Microbiology·Judith Berman
Nov 10, 2006·Molecular Biology of the Cell·Helen Court, Peter Sudbery
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
May 18, 2007·Annual Review of Microbiology·Malcolm Whiteway, Catherine Bachewich
Jun 2, 2007·Cell·Johannes L BosAlfred Wittinghofer

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Citations

Feb 12, 2014·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·M StaniszewskaW Kurzatkowski
Apr 17, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M StaniszewskaM Chudy
Sep 6, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Monika StaniszewskaHéctor M Mora-Montes
Mar 14, 2017·Future Microbiology·Monika StaniszewskaZbigniew Ochal
Oct 26, 2016·Bioorganic & Medicinal Chemistry·Edyta Łukowska-ChojnackaMonika Staniszewska

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