Abstract
Intrathecal (i.t.) injection of AM251, a cannabinoid 1 (CB(1)) receptor antagonist, into the spinal lumbar space of mice elicited a behavioral response consisting of biting and licking with a few scratchings. In this study, we investigated to determine whether i.t. AM251 could influence the activity of extracellular signal-regulated kinase-1 and -2 (ERK1/2), a mitogen-activated protein kinase (MAPK) in neuronal nitric oxide synthase (nNOS) and inducible NOS (iNOS) activation. The CB(1) receptor agonist ACEA, neurokinin 1 (NK(1)) receptor antagonists and NMDA receptor antagonists, inhibited i.t. AM251-induced behavioral response in a dose-dependent manner. The CB(2) receptor agonist, JWH-133 gave no effect on response elicited by i.t. AM251. Both non-selective NOS inhibitors, L-NAME and 7-NI, and N(ω)-propyl-L-arginine, a selective inhibitor of nNOS resulted in a dose-dependent inhibition of i.t. AM251-induced behavioral response. The selective iNOS inhibitor, 1400W, in relatively large doses, inhibited in a non dose-dependent manner. The i.t. injection of AM251 produced a definite activation of ERK1/2 in the lumbar dorsal spinal cord. Behavioral experiments showed that U0126, a MAPK/ERK kinase (MEK) inhibitor, dose-dependently ...Continue Reading
References
Dec 14, 1992·Neuroscience Letters·K F KittoG L Wilcox
Sep 1, 1987·Neuropharmacology·K TakahasiK Kisara
Dec 19, 1989·European Journal of Pharmacology·T SakuradaM Ohba
Jul 27, 1981·Brain Research·J L Hylden, G L Wilcox
Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
Feb 21, 1994·Brain Research·P LiJ P Figueroa
Mar 14, 1994·Neuroscience Letters·J H SteelJ M Polak
Aug 1, 1994·Neuroscience·W WuS H Snyder
Mar 14, 1995·European Journal of Pharmacology·Y KuraishiM Satoh
Feb 1, 1993·Pain·S T Meller, G F Gebhart
Sep 1, 1995·The European Journal of Neuroscience·V RadhakrishnanJ L Henry
Jun 1, 1996·Journal of the Neurological Sciences·Y ChoiJ R Tobin
Jan 1, 1996·Life Sciences·T SakuradaK Kisara
Sep 1, 1996·Neurochemistry International·T SakuradaY Abiko
Aug 29, 1997·The Journal of Biological Chemistry·M BouaboulaP Casellas
Dec 5, 1998·Brain Research·N M StrangmanJ M Walker
Sep 14, 1999·British Journal of Pharmacology·V Chapman
Nov 26, 1999·Nature Neuroscience·R R JiC J Woolf
Sep 16, 2000·The Journal of Physiology·E M Fitzgerald
Feb 27, 2001·Neurochemistry International·C SakuradaT Sakurada
Apr 27, 2001·Pain·K OkudaT Sakurada
Jan 12, 2002·British Journal of Pharmacology·I J Lever, M Malcangio
May 17, 2003·Journal of Neurophysiology·Hui-Juan HuRobert W Gereau
May 31, 2003·Canadian Journal of Physiology and Pharmacology·Clemente VásquezLeonardo Hernández
Oct 24, 2003·Molecular and Cellular Neurosciences·Isobel J LeverMarzia Malcangio
Mar 3, 2004·British Journal of Pharmacology·M SteffensT J Feuerstein
Sep 15, 2004·Pain·S M SweitzerD C Yeomans
Jul 28, 2005·The European Journal of Neuroscience·Devi Rani SagarVictoria Chapman
Dec 16, 2005·The AAPS Journal·Roger G Pertwee
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhi-Ye ZhuangRu-Rong Ji
Jan 20, 2007·The AAPS Journal·Andrea G Hohmann, Richard L Suplita
Mar 14, 2007·Neuropharmacology·Takaaki KomatsuTsukasa Sakurada
Jun 15, 2007·The European Journal of Neuroscience·Qingbo TangXiao-Ying Hua
Jan 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C D MunhozJ C Leza