The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter

Metallomics : Integrated Biometal Science
Melania D'OrazioAndrea Battistoni

Abstract

The ability of a large number of bacterial pathogens to multiply in the infected host and cause disease is dependent on their ability to express high affinity zinc importers. In many bacteria, ZnuABC, a transporter of the ABC family, plays a central role in the process of zinc uptake in zinc poor environments, including the tissues of the infected host. To initiate an investigation into the relevance of the zinc uptake apparatus for Pseudomonas aeruginosa pathogenicity, we have generated a znuA mutant in the PA14 strain. We have found that this mutant strain displays a limited growth defect in zinc depleted media. The znuA mutant strain is more sensitive than the wild type strain to calprotectin-mediated growth inhibition, but both the strains are highly resistant to this zinc sequestering antimicrobial protein. Moreover, intracellular zinc content is not evidently affected by inactivation of the ZnuABC transporter. These findings suggest that P. aeruginosa is equipped with redundant mechanisms for the acquisition of zinc that might favor P. aeruginosa colonization of environments containing low levels of this metal. Nonetheless, deletion of znuA affects alginate production, reduces the activity of extracellular zinc-containing...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·D EisenbergJ D Bell
Sep 1, 1968·Analytical Biochemistry·C A Knutson, A Jeanes
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·J WangS Jin
Apr 11, 1997·The Journal of Biological Chemistry·E KesslerD E Ohman
Jul 29, 1998·Molecular Microbiology·S I Patzer, K Hantke
Nov 7, 1998·The Journal of Biological Chemistry·E KesslerD E Ohman
Mar 10, 2001·Analytical Biochemistry·A R CaballeroR J O'Callaghan
Jan 5, 2002·BioTechniques·H P Schweizer, R Chuanchuen
Mar 28, 2003·Biochimica Et Biophysica Acta·Qinhong MaMilton H Saier
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina M PaninaMikhail S Gelfand
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianxin HeLaurence G Rahme
Nov 18, 2004·Biochimica Et Biophysica Acta·P Delepelaire
Jul 5, 2005·The Journal of Infectious Diseases·Alessandra BragonziGerd Doring
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeff G LeidAlyssa K Jeffers
Nov 4, 2006·Journal of Proteome Research·Claudia AndreiniAntonio Rosato
Mar 17, 2007·The Journal of Clinical Investigation·Yukihiro KanekoPradeep K Singh
Feb 5, 2008·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Gordon MacGregorAndrew P Greening
Feb 16, 2008·Science·Brian D CorbinEric P Skaar
Jun 21, 2008·American Journal of Respiratory and Critical Care Medicine·Robert D GrayAndrew P Greening
Dec 17, 2008·Nature Reviews. Microbiology·Kevin J Waldron, Nigel J Robinson
Jan 9, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Iain L LamontDavid W Reid
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Oded LewinsonDouglas C Rees
Aug 25, 2009·Accounts of Chemical Research·Claudia AndreiniAntonio Rosato
Jan 25, 2011·Molecular Microbiology·Crysten E Blaby-HaasValérie de Crécy-Lagard

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Citations

Sep 13, 2016·Annual Review of Genetics·Lauren D Palmer, Eric P Skaar
Oct 19, 2017·Pathogens and Disease·Cynthia Nau Cornelissen
Sep 13, 2017·Molecular Microbiology·Maria Chiara MastropasquaAndrea Battistoni
Sep 8, 2018·Metallomics : Integrated Biometal Science·Jelena HabjaničEva Freisinger
Apr 11, 2018·PloS One·Fernando A PagliaiGraciela L Lorca
Jun 22, 2017·Future Medicinal Chemistry·Andrea BattistoniAndrea Ilari
Sep 25, 2019·Infection and Immunity·Laura E HesseEric P Skaar
Aug 15, 2020·Frontiers in Microbiology·Jelena HabjaničEva Freisinger
Sep 1, 2017·FEMS Microbiology Letters·Corey B DaviesFlavia Huygens
Jan 11, 2019·Biochemical Society Transactions·Stephanie L Begg
Nov 3, 2021·Acta Crystallographica. Section D, Structural Biology·Annarita FiorilloAndrea Ilari

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