The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus

FEMS Microbiology Ecology
Magnus EllströmAnders Tunlid

Abstract

The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy an...Continue Reading

References

Nov 15, 1984·Analytical Biochemistry·S S Twining
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M H Saier
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Apr 18, 2002·Critical Reviews in Biotechnology·Stewart WhiteBrian McNeil
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Dec 19, 2003·Nucleic Acids Research·Rolf ApweilerLai-Su L Yeh
May 16, 2006·Trends in Plant Science·Michel ChalotAnnick Brun
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Jul 31, 2008·The New Phytologist·Eva LucicAnnick Brun-Jacob
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Nov 18, 2008·Fungal Genetics and Biology : FG & B·Judith K PollackMark R Marten
May 7, 2009·FEMS Microbiology Reviews·Amir SharonIdo Hatam
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 17, 2011·Nucleic Acids Research·Neil D RawlingsAlex Bateman
Nov 17, 2012·Microbiology·Melinda SzilágyiTamás Emri
Mar 22, 2013·Biotechnology for Biofuels·Anthony LevasseurBernard Henrissat
May 24, 2013·Applied Microbiology and Biotechnology·Benjamin M NitscheArthur F J Ram
Oct 1, 2013·Applied Microbiology and Biotechnology·Oliver Voigt, Stefanie Pöggeler
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Dec 25, 2013·Cell Research·Yuchen FengDaniel J Klionsky
Dec 20, 2014·The New Phytologist·Björn D Lindahl, Anders Tunlid

❮ Previous
Next ❯

Citations

Nov 6, 2015·The New Phytologist·Joseph J HendricksStephen D Pecot
Jan 4, 2018·The New Phytologist·Michiel Op De BeeckAnders Tunlid
May 12, 2019·Applied and Environmental Microbiology·Inger SkredeGry Alfredsen
Jun 1, 2018·International Journal of Microbiology·Gerald N PresleyJonathan S Schilling
Mar 17, 2019·Applied and Environmental Microbiology·Tao WangPer Persson
May 10, 2018·Frontiers in Microbiology·Vojtěch BenešPavel Kotrba
May 6, 2021·Natural Product Research·Jian-Hua LvYu Li
May 19, 2021·Bioorganic Chemistry·Jianhua LvYu Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
GPL14950
GSE54940

Methods Mentioned

BETA
Assay
fluorescence spectroscopy
fluorescence
PCA
transcriptomic profiling
deamination

Software Mentioned

SignalP
Isotig
blastp
NimbleScan
Omics Explorer

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.