PMID: 2120241Oct 1, 1990Paper

The carboxy-terminal domain of Gs alpha is necessary for anchorage of the activated form in the plasma membrane

The Journal of Cell Biology
Y AudigierJ Bockaert

Abstract

GTP-binding proteins which participate in signal transduction share a common heterotrimeric structure of the alpha beta gamma-type. In the activated state, the alpha subunit dissociates from the beta gamma complex but remains anchored in the membrane. The alpha subunits of several GTP-binding proteins, such as Go and Gi, are myristoylated at the amino terminus (Buss, J. E., S. M. Mumby, P. J. Casey, A. G. Gilman, and B. M. Sefton. 1987. Proc. Natl. Acad. Sci. USA. 84:7493-7497). This hydrophobic modification is crucial for their membrane attachment. The absence of fatty acid on the alpha subunit of Gs (Gs alpha), the protein involved in adenylate cyclase activation, suggests a different mode of anchorage. To characterize the anchoring domain of Gs alpha, we used a reconstitution model in which posttranslational addition of in vitro-translated Gs alpha to cyc- membranes (obtained from a mutant of S49 cell line which does not express Gs alpha) restores the coupling between the beta-adrenergic receptor and adenylate cyclase. The consequence of deletions generated by proteolytic removal of amino acid sequences or introduced by genetic removal of coding sequences was determined by analyzing membrane association of the proteolyzed or...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T L JonesA M Spiegel
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Nov 13, 1987·Biochemical and Biophysical Research Communications·B EideA Spiegel
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J E BussB M Sefton
May 12, 1988·Nature·E J Neer, D E Clapham

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Citations

Jan 1, 1994·Protein Science : a Publication of the Protein Society·E J Neer
Mar 1, 1994·Molecular and Cellular Endocrinology·M CormontY Le Marchand-Brustel
Jun 1, 1991·Current Opinion in Neurobiology·J Bockaert
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L JournotY Audigier
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M E LinderS M Mumby
Aug 10, 2000·The Journal of Biological Chemistry·M KlemkeW B Huttner
May 26, 1994·Biochimica Et Biophysica Acta·T YagamiY Mizushima
Sep 11, 1991·Trends in Biochemical Sciences·A M SpiegelW F Simonds

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