The carboxy-terminal transactivation domain of Oct-4 acquires cell specificity through the POU domain.

Molecular and Cellular Biology
A BrehmH Schöler

Abstract

The POU transcription factor Oct-4 is expressed in totipotent and pluripotent cells of the early mouse embryo and the germ cell lineage. Transactivation capacities of regions flanking the DNA binding domain of Oct-4 were analyzed in undifferentiated and differentiated cell lines. The amino- and carboxy-terminal regions (N domain and C domain) fused to the Gal4 DNA binding domain both functioned as transactivation domains in all cell lines tested. However, the C domain failed to activate transcription in some cell lines in the context of the native protein. The underlying regulatory mechanism appears to involve the POU domain of Oct-4 and can discriminate between different POU domains, since constructs in which the C domain was instead fused to the POU domain of Pit-1 were again equally active in all cell lines. These results indicate that the C domain is subject to cell-type-specific regulation mediated by the Oct-4 POU domain. Phosphopeptide analysis revealed that the cell-type-specific difference of C-domain activity correlates with a difference in Oct-4 phosphorylation status. Since Oct-4 is expressed in a variety of distinct cell types during murine embryogenesis, these results suggest an additional regulatory mechanism for...Continue Reading

References

Jul 1, 1992·Molecular and Cellular Biology·A AnnweilerT Wirth
Mar 29, 1990·Nature·H R SchölerP Gruss
Nov 1, 1991·Mechanisms of Development·Y I YeomK Artzt
Oct 1, 1991·Trends in Genetics : TIG·H R Schöler
Mar 2, 1989·Nature·J W Lillie, M R Green
Aug 11, 1989·Nucleic Acids Research·P MatthiasW Schaffner
Dec 8, 1988·Nature·R A Sturm, W Herr
Oct 6, 1988·Nature·I SadowskiM Ptashne
Sep 5, 1988·Journal of Molecular Biology·P SchillerR Voellmy
Jul 23, 1982·Science·S L McKnight, R Kingsbury
Apr 1, 1995·Current Opinion in Genetics & Development·S J Triezenberg
Jan 26, 1995·Nature·M GstaigerC M Hovens
Mar 25, 1994·Nucleic Acids Research·I Sylvester, H R Schöler
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E S MonukiG Lemke
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M WegnerM G Rosenfeld
Apr 29, 1993·Biochimica Et Biophysica Acta·C P Verrijzer, P C Van der Vliet

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Citations

May 19, 2009·Biochemical and Biophysical Research Communications·Hye-Young LimJae-Hwan Kim
May 27, 2005·Stem Cells·Jungwoon LeeJungho Kim
Jul 11, 2009·Stem Cells·Gretchen A BaltusShilpa Kadam
Nov 23, 2007·Stem Cells·Tobias CantzHans R Schöler
Dec 6, 2008·The Prostate·Paula SotomayorWendy J Huss
Feb 23, 2011·The Journal of Pathology·Shidou ZhaoAijun Hao
Oct 23, 2013·Biochimica Et Biophysica Acta·Stepan JerabekVlad Cojocaru
Jun 12, 2012·Stem Cell Research·Chang-long GuoLiu Wang
Dec 12, 2003·Cancer Cell·Sharon GidekelEli Pikarsky
Feb 17, 2015·Mechanisms of Development·Maria Lucia ScaldaferriMassimo De Felici
Oct 8, 2008·BMC Developmental Biology·Maurizio ZuccottiJames Adjaye
Mar 4, 2006·FEBS Letters·Satoshi KishigamiTeruhiko Wakayama
Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A BrehmH R Schöler
Nov 20, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M PesceH R Schöler
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Justin BrumbaughJames A Thomson
Mar 1, 2000·Molecular Reproduction and Development·M Pesce, H R Schöler
Sep 20, 2017·Glycobiology·Sandii ConstableLance Wells
Sep 3, 1999·The Journal of Biological Chemistry·J HildesheimJ C Vogel
Nov 25, 2017·Experimental & Molecular Medicine·Eun Kyoung DoJae Ho Kim
Sep 22, 2001·Cell Structure and Function·H Niwa
Feb 27, 2014·Cellular Reprogramming·Suresh RamakrishnaKwang-Hyun Baek
Oct 22, 2008·Molecular Reproduction and Development·Miyuri KawasumiKazuya Matsumoto
Apr 4, 2008·Proteomics·Miguel BarthéléryKent E Vrana
Sep 14, 2018·Oncogene·Sheng-Chieh LinCheng-Wen Wu
Dec 9, 2009·The Biochemical Journal·Jungwoon LeeJungho Kim
Jan 17, 2003·Cell Research·Guang Jin PanDuanqing Pei
May 15, 1998·Nature·J A Lefstin, K R Yamamoto
Sep 5, 2006·The Journal of Biological Chemistry·Jungwoon LeeJungho Kim
May 26, 2007·The Journal of Biological Chemistry·Fang WeiMichael L Atchison
Jun 4, 2019·International Journal of Molecular Sciences·Dian WangWeiwei Zhang
Nov 2, 2001·Molecular and Cellular Biology·T EzashiR M Roberts

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