The carboxy terminus of p53 mimics the polylysine effect of protein kinase CK2-catalyzed MDM2 phosphorylation

Oncogene
Barbara GuerraO G Issinger

Abstract

The oncogene product MDM2 can be phosphorylated by protein kinase CK2 in vitro 0.5-1 mol of phosphate were incorporated per mol MDM2 protein. The catalytic subunit of protein kinase CK2 (alpha-subunit) catalyzed the incorporation of twice as much phosphate into the MDM2 protein as it was obtained with the holoenzyme. Polylysine stimulated MDM2 phosphorylation by CK2 holoenzyme threefold in contrast to the alpha-subunit-catalyzed MDM2 phosphorylation which was reduced by about 66% when polylysine was added. Full length p53, but also a peptide representing a C-terminal fragment of the tumor suppressor gene product p53 (amino acids 264-393 which also harbors the CK2beta interaction site at amino acids 287-340) mimicked the polylysine effect in all respects, ie. stimulation of phosphate incorporation by CK2 holoenzyme and inhibition in the presence of the catalytic CK2 alpha-subunit. Stimulation by p53(264-393) was on the average close to twofold and inhibition in the case of the alpha-subunit-catalyzed MDM2 phosphorylation was about 40%. Phosphorylation of MDM2 by CK2 holoenzyme in the presence of the p21(WAF1/CIP1), known to be a potent inhibitor of cyclin-dependent protein kinases, also led to a significant reduction of phosphat...Continue Reading

Citations

Feb 17, 2001·Journal of Cellular Biochemistry·N SchusterM Montenarh
Oct 27, 2010·Cell and Tissue Research·Mathias Montenarh
May 6, 2003·Protein Expression and Purification·Marta RieraBarbara Guerra
Nov 21, 2007·Oncogene·S LehnertM Montenarh
Nov 24, 1999·European Journal of Biochemistry·C GötzM Montenarh
Jul 12, 2002·The EMBO Journal·Zehavit GoldbergYgal Haupt
Mar 14, 2003·Journal of Virology·Maria D KoffaJ Barklie Clements
Feb 19, 2000·Biochemical and Biophysical Research Communications·C GötzM Montenarh
May 1, 2001·Biochemical and Biophysical Research Communications·M AbabnehM Montenarh
Aug 26, 2014·Molecular and Cellular Biochemistry·Gerda M HübnerOlaf-Georg Issinger
Jun 16, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yukimasa TakeishiToshiki Tsurimoto
Mar 1, 2012·Molecular Cell·Svetlana V KhoronenkovaGrigory L Dianov
May 2, 2002·Molecular Cell·Koji OkamotoCarol Prives
Oct 31, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Andreas GratzJoachim Jose
Jul 26, 2002·Oncogene·Eberhard SchneiderMathias Montenarh
Sep 15, 2016·Surgical Neurology International·Masatoshi YunokiKimihiro Yoshino
Apr 18, 2002·Oncogene·Margaret AshcroftKaren H Vousden
May 22, 2001·The Journal of Biological Chemistry·T Zhang, C Prives
Apr 18, 2003·The Journal of Biological Chemistry·Diane H SongDavid C Seldin
Jan 1, 1998·Journal of Cellular Biochemistry·C C Allende, J E Allende
Dec 13, 2005·Molecular and Cellular Biochemistry·Karsten Niefind, Olaf-Georg Issinger
Jul 14, 2011·Molecular and Cellular Biochemistry·Andreas GratzJoachim Jose
Dec 13, 2005·Molecular and Cellular Biochemistry·Claudia GötzMathias Montenarh
Apr 14, 2021·Proceedings of the National Academy of Sciences of the United States of America·Abdelhalim LoukilSarah C Goetz

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