The carboxyl-terminal domain of large T antigen rescues SV40 host range activity in trans independent of acetylation

Virology
Danielle L Poulin, J A DeCaprio

Abstract

The host range activity of SV40 has been described as the inability of mutant viruses with deletions in the C terminal region of large T Ag to replicate in certain types of African green monkey kidney cells. We constructed new mutant viruses expressing truncated T Ag proteins and found that these mutant viruses exhibited the host range phenotype. The host range phenotype was independent of acetylation of T Ag at lysine 697. Co-expression of the C terminal domain of T Ag (aa 627-708) in trans increased both T Ag and VP1 mRNA as well as protein levels for host range mutant viruses in the restrictive cell type. In addition, the T Ag 627-708 fragment promoted the productive lytic infection of host range mutant viruses in the nonpermissive cell type. The carboxyl-terminal region of T Ag contains a biological function essential for the SV40 viral life cycle.

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Citations

Nov 6, 2007·Journal of Virology·Vojtech SrollerJanet S Butel
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Colin R ParrishPeter Daszak
Mar 20, 2009·Journal of Virology·Cathal MahonWilliam R Folk
Apr 12, 2008·Journal of Virological Methods·Rebecca B KatzmanKathleen Rundell
Mar 12, 2010·Journal of Molecular Biology·Isabel CuestaJose María Carazo
Nov 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Megan H OrzalliDavid M Knipe
Oct 20, 2018·Journal of Virology·Roxana M TarnitaJames A DeCaprio
Dec 29, 2020·EMBO Reports·Minghua NieMichael N Boddy

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