PMID: 8970944Dec 1, 1996Paper

The carboxyl terminus of the human foamy virus Gag protein contains separable nucleic acid binding and nuclear transport domains

Journal of Virology
S F YuM L Linial

Abstract

The Gag protein of human foamy virus (HFV) lacks Cys-His boxes present in the nucleocapsid (NC) domains of other retroviruses; instead it contains three glycine-arginine-rich motifs (GR boxes). We have expressed the carboxyl end of HFV Gag containing the GR boxes (the NC domain equivalent) and analyzed its nucleic acid binding properties. Our results show that the NC domain of HFV Gag binds with high affinity to both RNA and DNA, in a sequence-independent manner, as determined by filter binding assays. Analysis of a mutant containing a heterologous sequence in place of GR box I indicates that this motif is required for nucleic acid binding and for viral replication. A mutant in GR box II still binds to RNA and DNA in vitro, but virus containing this mutation does not replicate and no nuclear staining of the Gag protein is found in transfected cells. Surprisingly, a revertant from this mutant that completely lacks GR box II and exhibits very little nuclear transport of Gag can readily replicate in tissue culture. This finding thus provides a direct evidence that although the sequences in GR box II can serve as a nuclear transport signal, they are not required for HFV replication and it is unlikely that nuclear localization of Ga...Continue Reading

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Citations

May 20, 2004·British Journal of Cancer·H LiE A Engels
Sep 15, 2005·Journal of Virology·Marc CartellieriAxel Rethwilm
Dec 21, 2007·Journal of Virology·Liza S Z LarsenSuzanne Sandmeyer
Sep 28, 2012·Journal of Virology·Réjane RuaAntoine Gessain
Feb 11, 2015·Bioscience, Biotechnology, and Biochemistry·Md Khadem AliCha-Gyun Shin
Jul 5, 2005·Journal of Virology·Jacqueline Lehmann-CheAli Saïb
Jul 17, 2008·Traffic·Joelle Tobaly-TapieroAli Saïb
Oct 26, 2010·Journal of Neurovirology·Yingying ZhangXiaohua He
May 7, 2010·Medical Microbiology and Immunology·Axel Rethwilm
Apr 10, 1997·AIDS Research and Human Retroviruses·N FischerS Niewiesk
Aug 17, 2004·Journal of Virology·Carolyn R Stenbak, Maxine L Linial
Nov 22, 2000·The Journal of General Virology·H ImrichA Rethwilm
Mar 23, 2013·Viruses·Eun-Gyung LeeMaxine L Linial
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Sep 18, 2009·Journal of Virology·Nadejda Beliakova-BethellSuzanne Sandmeyer
Apr 7, 2016·Cell Communication and Signaling : CCS·Aino I JärvelinAlfredo Castello
Sep 3, 2016·Viruses·Martin V Hamann, Dirk Lindemann
Feb 7, 2001·Journal of Virology·J Tobaly-TapieroA Saïb
Dec 17, 2010·Journal of Virology·Jochen BodemAxel Rethwilm
Jul 23, 2013·Viruses·Md Alamgir HossainCha-Gyun Shin
Feb 4, 2011·Journal of Virology·Daniel MatthesAxel Rethwilm
Jul 5, 2005·Journal of Virology·Lisa Z ScheifeleLeslie J Parent

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