Abstract
The cytokine interleukin 1ss (IL-1ss) and the bradykinin receptors 1 (B1R) and 2 (B2R) are known to be upregulated in the ischemic heart. In the present study we investigated whether or not there is a causal link between these entities. Further we investigated whether or not pharmacological inhibition of IL-1ss release affects B1R and B2R regulation as well as left ventricular (LV) function in an in vivo rat model of myocardial infarction (MI). B1R and B2R mRNA levels were determined in cultured rat cardiomyocytes, aortic smooth muscle cells and cardiac fibroblasts (n=6 per group) under basal conditions, and after incubation of IL-1ss (40, 400 and 4000 pg/ml). Also, MI was induced in male Sprague-Dawley rats by ligation of the left descending coronary artery. Rats were treated with the interleukin converting enzyme inhibitor (ICEI) pralnacasan (50 mg/kg/day), or with a placebo. Three weeks after induction of MI, LV function was assessed using a 1.4 Millar TIP-catheter. Cardiac expressions of B1R and B2R mRNA were measured using ribonuclease-protection assays. Under basal conditions, both B1R and B2R were expressed in cardiomyocytes and smooth muscle cells, but not in cardiac fibroblasts. IL-1ss cultivation led only in cardiomyo...Continue Reading
References
Sep 1, 1992·The Journal of Experimental Medicine·D J KesslerG E Sonenshein
Jun 19, 1991·European Journal of Pharmacology·T M KeravisJ C Stoclet
Mar 15, 1988·European Journal of Pharmacology·Y FujiwaraH I Yamamura
May 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·I KleinM Gondek
Jan 6, 1996·Lancet·A Blum
Feb 17, 1999·Journal of Molecular and Cellular Cardiology·N G FrangogiannisM L Entman
Dec 22, 1999·Immunopharmacology·J RehbockP Buchinger
Jan 15, 2000·Biochimica Et Biophysica Acta·K YayamaH Okamoto
Jun 14, 2000·European Journal of Pharmacology·S B PhagooG M Burgess
Mar 3, 2001·Angiology·Y BalbayS Korkmaz
Dec 20, 2002·International Immunopharmacology·Frank SpillmannCarsten Tschöpe
Feb 14, 2004·Cardiovascular Research·Carsten TschöpeThomas Walther
Feb 9, 2005·Hypertension·Jiang XuXiao-Ping Yang
May 30, 2006·The Annals of Thoracic Surgery·Tao-Sheng LiKimikazu Hamano
Jul 18, 2006·Current Medicinal Chemistry·Nikolaos G Frangogiannis
Oct 7, 2006·European Journal of Pharmacology·Matthias KochCarsten Tschöpe
Dec 5, 2006·Current Opinion in Nephrology and Hypertension·Alexander RiadCarsten Tschöpe
May 3, 2007·Diabetes·Dirk WestermannCarsten Tschöpe
Citations
Jul 17, 2008·Biological Chemistry·Carsten Tschöpe, Dirk Westermann
Nov 26, 2010·Biochemical and Biophysical Research Communications·K RichesC Peers
Aug 23, 2011·Mechanisms of Ageing and Development·Anna AtlanteSalvatore Passarella
Jan 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexander RiadCarsten Tschöpe
Sep 11, 2021·European Journal of Pharmacology·Jinchao SongBinhai Cong