The caspase-1 inhibitor CARD18 is specifically expressed during late differentiation of keratinocytes and its expression is lost in lichen planus

Journal of Dermatological Science
Haihong QinE Tschachler

Abstract

CARD18 contains a caspase recruitment domain (CARD) via which it binds to caspase-1 and thereby inhibits caspase-1-mediated activation of the pro-inflammatory cytokine interleukin (IL)-1β. To determine the expression profile and the role of CARD18 during differentiation of keratinocytes and to compare the expression of CARD18 in normal skin and in inflammatory skin diseases. Human keratinocytes were induced to differentiate in monolayer and in 3D skin equivalent cultures. In some experiments, CARD18-specific siRNAs were used to knock down expression of CARD18. CARD18 mRNA levels were determined by quantitative real-time PCR, and CARD18 protein was detected by Western blot and immunofluorescence analyses. In situ expression was analyzed in skin biopsies obtained from healthy donors and patients with psoriasis and lichen planus. CARD18 mRNA was expressed in the epidermis at more than 100-fold higher levels than in any other human tissue. Within the epidermis, CARD18 was specifically expressed in the granular layer. In vitro CARD18 was strongly upregulated at both mRNA and protein levels in keratinocytes undergoing terminal differentiation. In skin equivalent cultures the expression of CARD18 was efficiently suppressed by siRNAs w...Continue Reading

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Citations

Jun 5, 2018·Experimental Dermatology·Leopold Eckhart, Erwin Tschachler
Sep 24, 2020·International Journal of Molecular Sciences·Savita DeviAndrea Dorfleutner
Mar 20, 2021·Photodermatology, Photoimmunology & Photomedicine·Ziting TangShengbo Yang
May 1, 2021·International Journal of Molecular Sciences·Judit Danis, Mark Mellett
Jul 4, 2021·The Journal of Investigative Dermatology·Evelyn KelemenMárta Széll

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