The cassava (Manihot esculenta Crantz) root proteome: protein identification and differential expression

Proteomics
Jeanne SheffieldSixue Chen

Abstract

Using high-resolution 2-DE, we resolved proteins extracted from fibrous and tuberous root tissues of 3-month-old cassava plants. Gel image analysis revealed an average of 1467 electrophoretically resolved spots on the fibrous gels and 1595 spots on the tuberous gels in pH 3-10 range. Protein spots from both sets of gels were digested with trypsin. The digests were subjected to nanoelectrospray quadrupole TOF tandem mass analysis. Currently, we have obtained 299 protein identifications for 292 gel spots corresponding to 237 proteins. The proteins span various functional categories from energy, primary and secondary metabolism, disease and defense, destination and storage, transport, signal transduction, protein synthesis, cell structure, and transcription to cell growth and division. Gel image analysis has shown unique, as well as up- and down-regulated proteins, present in the tuberous and the fibrous tissues. Quantitative and qualitative analysis of the cassava root proteome is an important step towards further characterization of differentially expressed proteins and the elucidation of the mechanisms underlying the development and biological functions of the two types of roots.

References

Jan 19, 2000·Trends in Genetics : TIG·J R Yates
Jan 10, 2002·Trends in Biotechnology·B R HerbertK L Williams
Feb 16, 2002·Science·Mark J AustinJane E Parker
May 22, 2002·Journal of Experimental Botany·Dorothee U KloosReinhard Hehl
Aug 8, 2002·Proteomics·William S HancockPaul Shieh
Sep 11, 2002·Rapid Communications in Mass Spectrometry : RCM·Valérie GabelicaEdwin De Pauw
Feb 27, 2003·Plant Foods for Human Nutrition·C R B De SouzaE S Gander
Jan 5, 2005·Plant Molecular Biology·Nigel TaylorPeng Zhang

❮ Previous
Next ❯

Citations

Dec 28, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ahmed J AfzalDavid A Lightfoot
Oct 13, 2007·Plant Cell Reports·Sang Gon KimKyu Young Kang
Apr 13, 2007·Plant Molecular Biology·Donald LivingstoneNiels C Nielsen
Jun 27, 2013·Journal of Proteome Research·Le YangHaiying Li
Oct 5, 2010·The Journal of Pharmacology and Experimental Therapeutics·Wenjun LiPeter W Stacpoole
Apr 30, 2011·Annual Review of Plant Biology·Richard SayrePeng Zhang
Mar 20, 2013·Biochimica Et Biophysica Acta·Joel W GrayTimothy P Devarenne
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Jeung Joo LeeSang-Soo Kwak
Oct 29, 2015·Data in Brief·Yuliang ZhangGuohua Yin
Jan 10, 2012·Current Opinion in Biotechnology·Elisa Leyva-GuerreroRichard T Sayre
Nov 9, 2015·Journal of Plant Research·Maliwan NaconsieJarunya Narangajavana
Sep 29, 2009·Journal of Proteomics·Haiying LiSixue Chen
Jul 25, 2009·Journal of Proteomics·Sophie AlvarezSixue Chen
Dec 9, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sophie AlvarezSixue Chen
Jan 10, 2009·Proteomics·Warren AlbertinHervé Thiellement
Jul 27, 2007·The FEBS Journal·Jean-Emmanuel SarryPhilip A Rea
Sep 15, 2006·Proteomics·Sixue Chen, Alice C Harmon
Nov 19, 2010·The New Phytologist·Anuphon Laohavisit, Julia M Davies
Mar 17, 2016·Journal of Agricultural and Food Chemistry·Gabriela Justamante Hendel SchmitzJoão Roberto Oliveira Nascimento
Mar 26, 2011·The Plant Journal : for Cell and Molecular Biology·Judith OwitiHervé Vanderschuren
Dec 3, 2014·Ecotoxicology and Environmental Safety·Hui-Xi ZouXiu-Feng Yan
Jun 30, 2015·Plant Molecular Biology·Punchapat SojikulJarunya Narangajavana
Nov 16, 2017·International Journal of Molecular Sciences·Yuan YaoJian-Chun Guo
Dec 21, 2017·Tropical Animal Health and Production·Paula de Aguiar SilvaWilson Souza Nunes
Jan 13, 2018·International Journal of Molecular Sciences·Long-Bin ShenYan Yang
Dec 10, 2017·Scientific Reports·Ratana ThanasomboonTreenut Saithong
Nov 25, 2016·Plant Molecular Biology Reporter·Feifei AnSongbi Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.