The catalytic activity, but not receptor binding, of sPLA2s plays a critical role for neurite outgrowth induction in PC12 cells

Brain Research
Satoru NakashimaManabu Arioka

Abstract

We previously showed that fungal secretory phospholipase A2 (sPLA2) induces neurite formation in PC12 cells in an L-type Ca2+ channel activity-dependent manner. In this study we compared neurite-inducing activity of different sPLA2s, including bee venom sPLA2 (bvPLA2), and found that it correlated with the ability of each sPLA2 to release fatty acids from live PC12 cells. Consistently, using several mutants of bvPLA2, we found that the enzymatic activity rather than the binding activity to the putative N-type receptor for neurotoxic sPLA2s is the critical determinant for the neuritogenic response. These results imply that the neurite outgrowth is elicited by the messenger(s) produced upon degradation of membrane phospholipids.

References

Jan 31, 1998·The Journal of Biological Chemistry·K HanasakiH Arita
May 14, 1999·Trends in Pharmacological Sciences·G Lambeau, M Lazdunski
Mar 2, 2002·Journal of Biochemistry·Makoto Murakami, Ichiro Kudo
Mar 19, 2002·The Journal of Biological Chemistry·Masanori SugiyamaHideo Misaki
Mar 7, 2003·Bioscience, Biotechnology, and Biochemistry·Satoru NakashimaKatsuhiko Kitamoto
Sep 12, 2003·The Biochemical Journal·Satoru NakashimaManabu Arioka

❮ Previous
Next ❯

Citations

Oct 27, 2009·Neuromolecular Medicine·Grace Y SunAgnes Simonyi
Mar 20, 2010·Molecular Neurobiology·Gianfrancesco GoracciVincenza Nardicchi
Oct 12, 2013·Molecular Neurobiology·Tatsurou YagamiHiromi Koma
May 3, 2005·FEBS Letters·Manabu AriokaKatsuhiko Kitamoto
Nov 22, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Elen Cristina Teizem LanducciLuis Alberto Ponce-Soto
Jul 23, 2009·Toxicon : Official Journal of the International Society on Toxinology·Alexey V OsipovYuri N Utkin
May 6, 2010·Journal of Zhejiang University. Science. B·Li-Rong ShenDuo Li
Mar 23, 2005·The Journal of Biological Chemistry·Seiko MasudaIchiro Kudo
Aug 24, 2005·Archives of Insect Biochemistry and Physiology·Jiang-Hong LiJia-An Cheng
Oct 21, 2004·Blood·Reinhold RamonerMartin Thurnher
Mar 13, 2018·Frontiers in Pharmacology·Jessica M de SouzaFabiola M Ribeiro
Sep 18, 2007·The Biochemical Journal·Seiko MasudaMakoto Murakami
Nov 4, 2006·Biochimica Et Biophysica Acta·Gerwald A KöhlerSantosh Nigam
May 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·René D MartinsHelena S A Monteiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.