PMID: 9177177Jun 10, 1997Paper

The catalytic domain of lambda site-specific recombinase

Proceedings of the National Academy of Sciences of the United States of America
Radhakrishna TirumalaiA Landy

Abstract

The Escherichia coli phage lambda integrase protein (Int) belongs to the large Int family of site-specific recombinases. It is a heterobivalent DNA binding protein that makes use of a high energy covalent phosphotyrosine intermediate to catalyze integrative and excisive recombination at specific chromosomal sites (att sites). A 293-amino acid carboxy-terminal fragment of Int (C65) has been cloned, characterized, and used to further dissect the protein. From this we have cloned and characterized a 188-amino acid, protease-resistant, carboxy-terminal fragment (C170) that we believe is the minimal catalytically competent domain of Int. C170 has topoisomerase activity and converts att suicide substrates to the covalent phosphotyrosine complexes characteristic of recombination intermediates. However, it does not show efficient binding to att site DNA in a native gel shift assay. We propose that lambda Int consists of three functional and structural domains: residues 1-64 specify recognition of "arm-type" DNA sequences distant from the region of strand exchange; residues 65-169 contribute to specific recognition of "core-type" sequences at the sites of strand exchange and possibly to protein-protein interactions; and residues 170-356...Continue Reading

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Citations

Dec 23, 2003·Journal of Molecular Biology·Amy C Groth, Michele P Calos
Dec 4, 2002·Journal of Molecular Biology·Michael TekleSimone E Nunes-Düby
Dec 4, 2002·Journal of Molecular Biology·Dibyendu SarkarArthur Landy
Jan 1, 2003·Journal of Molecular Biology·Mikhail Kolot, Ezra Yagil
May 28, 2003·Journal of Molecular Biology·Srisunder SubramaniamMark P Foster
Nov 13, 2002·Biochimica Et Biophysica Acta·Henri De GreveJean Pierre Hernalsteens
Dec 6, 2002·Nucleic Acids Research·Robert A KazmierczakJeffrey F Gardner
Jun 12, 2012·Acta Crystallographica. Section D, Biological Crystallography·Zhengyan ZhanLi Huang
Jun 10, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hari B KamaduraiMark P Foster
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·David WarrenArthur Landy
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan M WojciakRobert T Clubb
Sep 8, 2001·Journal of Molecular Biology·H FerreiraL Arciszewska
Jan 22, 2005·Virology·Clara FrumerieElisabeth Haggård-Ljungquist
Mar 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Milton H Saier
Feb 6, 2002·The Journal of Biological Chemistry·Margaret J KovachArthur Landy
Jun 22, 2002·The Journal of Biological Chemistry·Zheng Yang, James J Champoux
Nov 5, 1999·Journal of Bacteriology·M V FranciaJ M García Lobo
Jun 26, 1998·Journal of Molecular Biology·R S TirumalaiA Landy
Jun 25, 2015·Microbiology Spectrum·Arthur Landy
Jul 31, 2003·Molecular Cell·Hideki AiharaTom Ellenberger

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