The catalytic machinery of a key enzyme in amino Acid biosynthesis.

Journal of Amino Acids
Ronald E ViolaAlexander G Pavlovsky

Abstract

The aspartate pathway of amino acid biosynthesis is essential for all microbial life but is absent in mammals. Characterizing the enzyme-catalyzed reactions in this pathway can identify new protein targets for the development of antibiotics with unique modes of action. The enzyme aspartate β-semialdehyde dehydrogenase (ASADH) catalyzes an early branch point reaction in the aspartate pathway. Kinetic, mutagenic, and structural studies of ASADH from various microbial species have been used to elucidate mechanistic details and to identify essential amino acids involved in substrate binding, catalysis, and enzyme regulation. Important structural and functional differences have been found between ASADHs isolated from these bacterial and fungal organisms, opening the possibility for developing species-specific antimicrobial agents that target this family of enzymes.

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Citations

Oct 8, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Bharani ThangaveluRonald Viola
Jan 8, 2015·Journal of Biomolecular Structure & Dynamics·Rajender KumarP V Bharatam
May 31, 2014·Journal of Biomolecular Structure & Dynamics·Rajender KumarP V Bharatam
Nov 4, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Gopal Dahal, Ronald E Viola
Jan 4, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Gopal P Dahal, Ronald E Viola
Jan 18, 2013·The Journal of Biological Chemistry·Ulrike DemmerUlrich Ermler

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.