The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA

Journal of Bacteriology
Richard Wargachuk, Gregory T Marczynski

Abstract

It is not known how diverse bacteria regulate chromosome replication. Based on Escherichia coli studies, DnaA initiates replication and the homolog of DnaA (Hda) inactivates DnaA using the RIDA (regulatory inactivation of DnaA) mechanism that thereby prevents extra chromosome replication cycles. RIDA may be widespread, because the distantly related Caulobacter crescentus homolog HdaA also prevents extra chromosome replication (J. Collier and L. Shapiro, J Bacteriol 191:5706-5715, 2009, http://dx.doi.org/10.1128/JB.00525-09). To further study the HdaA/RIDA mechanism, we created a C. crescentus strain that shuts off hdaA transcription and rapidly clears HdaA protein. We confirm that HdaA prevents extra replication, since cells lacking HdaA accumulate extra chromosome DNA. DnaA binds nucleotides ATP and ADP, and our results are consistent with the established E. coli mechanism whereby Hda converts active DnaA-ATP to inactive DnaA-ADP. However, unlike E. coli DnaA, C. crescentus DnaA is also regulated by selective proteolysis. C. crescentus cells lacking HdaA reduce DnaA proteolysis in logarithmically growing cells, thereby implicating HdaA in this selective DnaA turnover mechanism. Also, wild-type C. crescentus cells remove all Dn...Continue Reading

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Citations

Feb 13, 2016·Current Opinion in Microbiology·Justine Collier
Jun 3, 2016·Plasmid·Anna KarlowiczIgor Konieczny
Sep 27, 2016·Molecular Microbiology·Jing LiuPeter Chien
Feb 1, 2017·Nature Reviews. Microbiology·Régis HallezPatrick H Viollier
Dec 13, 2017·Frontiers in Microbiology·Julia E Grimwade, Alan C Leonard
Dec 3, 2016·Microbiology and Molecular Biology Reviews : MMBR·Revathy RamachandranDhruba Chattoraj
Oct 6, 2018·Molecular Microbiology·Jing LiuPeter Chien
Nov 13, 2019·Molecular Microbiology·Malgorzata Nowaczyk-CieszewskaAnna Magdalena Zawilak-Pawlik
Sep 10, 2019·Genes & Genetic Systems·Shogo Ozaki
Jun 6, 2019·Nature Reviews. Microbiology·Rodrigo Reyes-Lamothe, David J Sherratt
Jan 11, 2019·Biochemical Society Transactions·Antonio Frandi, Justine Collier
Sep 27, 2019·Frontiers in Microbiology·Alan C LeonardJulia E Grimwade
Mar 8, 2021·Current Opinion in Microbiology·François BeaufayRégis Hallez
Jun 19, 2021·Frontiers in Microbiology·Inoka P MenikpuragePaola E Mera

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