The caveolae-associated coiled-coil protein, NECC2, regulates insulin signalling in Adipocytes

Journal of Cellular and Molecular Medicine
Andrés TrávezMaría M Malagón

Abstract

Adipocyte dysfunction in obesity is commonly associated with impaired insulin signalling in adipocytes and insulin resistance. Insulin signalling has been associated with caveolae, which are coated by large complexes of caveolin and cavin proteins, along with proteins with membrane-binding and remodelling properties. Here, we analysed the regulation and function of a component of caveolae involved in growth factor signalling in neuroendocrine cells, neuroendocrine long coiled-coil protein-2 (NECC2), in adipocytes. Studies in 3T3-L1 cells showed that NECC2 expression increased during adipogenesis. Furthermore, NECC2 co-immunoprecipitated with caveolin-1 (CAV1) and exhibited a distribution pattern similar to that of the components of adipocyte caveolae, CAV1, Cavin1, the insulin receptor and cortical actin. Interestingly, NECC2 overexpression enhanced insulin-activated Akt phosphorylation, whereas NECC2 downregulation impaired insulin-induced phosphorylation of Akt and ERK2. Finally, an up-regulation of NECC2 in subcutaneous and omental adipose tissue was found in association with human obesity and insulin resistance. This effect was also observed in 3T3-L1 adipocytes exposed to hyperglycaemia/hyperinsulinemia. Overall, the prese...Continue Reading

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Citations

Jul 30, 2019·Frontiers in Medicine·Xiaoming LianMaik Gollasch
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Maria DudãuMihail E Hinescu
Jul 3, 2021·International Journal of Molecular Sciences·Maria J IraburuCristina Montiel-Duarte

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Methods Mentioned

BETA
confocal microscopy
PCR
transfection
confocal
immunoprecipitation

Software Mentioned

STRING
Huygens Essential
Imaris
ImageJ
SVI
Image J
SPSS

Related Concepts

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