The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle

The Journal of Cell Biology
Harriet P LoR G Parton

Abstract

Dysfunction of caveolae is involved in human muscle disease, although the underlying molecular mechanisms remain unclear. In this paper, we have functionally characterized mouse and zebrafish models of caveolae-associated muscle disease. Using electron tomography, we quantitatively defined the unique three-dimensional membrane architecture of the mature muscle surface. Caveolae occupied around 50% of the sarcolemmal area predominantly assembled into multilobed rosettes. These rosettes were preferentially disassembled in response to increased membrane tension. Caveola-deficient cavin-1(-/-) muscle fibers showed a striking loss of sarcolemmal organization, aberrant T-tubule structures, and increased sensitivity to membrane tension, which was rescued by muscle-specific Cavin-1 reexpression. In vivo imaging of live zebrafish embryos revealed that loss of muscle-specific Cavin-1 or expression of a dystrophy-associated Caveolin-3 mutant both led to sarcolemmal damage but only in response to vigorous muscle activity. Our findings define a conserved and critical role in mechanoprotection for the unique membrane architecture generated by the caveolin-cavin system.

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Methods Mentioned

BETA
transgenic
electron tomography
light microscopy
confocal microscopy
PCR
proximity ligation assay
dissection
protein assay
Assay
transfection

Software Mentioned

ImageJ
Photoshop
DP Capture
SerialEM
IMOD
Etomo
NIS Elements
ZEN

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