The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains.

The Journal of Biological Chemistry
R HorukR W Doms

Abstract

Using a chemokine receptor model based on known receptor sequences, we identified several members of the seven transmembrane domain G-protein superfamily as potential chemokine receptors. The orphan receptor ChemR1, which has recently been shown to be a receptor for the CC chemokine I-309, scored very high in our model. We have confirmed that I-309, but not a number of other chemokines, can induce a transient Ca2+ flux in cells expressing CCR8. In addition, the human erythroleukemic cell line K562 responded chemotactically in a dose-responsive manner to this chemokine. Since several chemokine receptors have been shown to be required as coreceptors for HIV-1 infection, we asked whether human immunodeficiency virus type 1 (HIV-1) could efficiently utilize CCR8. Here we show that the CCR8 receptor can serve as a coreceptor for diverse T-cell tropic, dual-tropic, and macrophage-tropic HIV-1 strains and that I-309 was a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. Furthermore, we show by flow cytometry and immunohistochemistry that antibodies generated against the CCR8 receptor amino-terminal peptide cross-reacted with U-87 MG cells stably expressing CCR8, THP-1 cells, HL-60 cells, and human mono...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M D Miller, M S Krangel
Jan 1, 1991·Annual Review of Biochemistry·H G DohlmanR J Lefkowitz
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 23, 1996·Biochemical and Biophysical Research Communications·A ZaballosG Márquez
Feb 17, 1997·The Journal of Experimental Medicine·R I ConnorN R Landau
Jul 7, 1997·The Journal of Experimental Medicine·H L TiffanyP M Murphy
Jul 11, 1997·The Journal of Biological Chemistry·R S RoosB Moser

❮ Previous
Next ❯

Citations

May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S MahalingamP S Foster
Sep 21, 2000·Folia Microbiologica·L Jindrák, L Grubhoffer
Dec 30, 1998·Archives of Pharmacal Research·H Choe
Aug 5, 1998·International Journal of Clinical & Laboratory Research·J M WangJ J Oppenheim
Jun 7, 2005·Developmental and Comparative Immunology·Zhongxia LiEllen W Collisson
Nov 20, 2003·Journal of Virological Methods·Tatsunori SakamotoWerner E G Müller
Dec 5, 1998·Journal of Immunological Methods·J M WangS Su
Apr 23, 1999·Journal of Reproductive Immunology·J A HoxieT J Matthews
Jun 6, 2003·Bioorganic & Medicinal Chemistry·Wieslaw KazmierskiTerry Kenakin
Sep 7, 2001·Cytokine & Growth Factor Reviews·R Horuk
May 11, 1999·Drug Discovery Today·J Saunders, C M Tarby
May 19, 1998·Nature Medicine·J S Cairns, M P D'Souza
Mar 6, 2002·Clinical and Experimental Immunology·R ZamarchiA Amadori
Nov 26, 1999·Immunology and Cell Biology·S Mahalingam, G Karupiah
Jan 6, 2000·The Journal of Experimental Medicine·H R LüttichauT W Schwartz
Aug 10, 2000·AIDS Research and Human Retroviruses·R D BerkowitzJ M McCune
Feb 15, 2001·AIDS Research and Human Retroviruses·Y J ZhangJ P Moore
Dec 22, 1999·AIDS Research and Human Retroviruses·Y L HongF Z Chung
Nov 20, 2002·AIDS Patient Care and STDs·Ralf Geiben-Lynn
Mar 8, 2005·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Quan-Zhen LiLili Feng
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
Jul 10, 1999·Immunological Reviews·A Pelchen-MatthewsM Marsh
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Jun 25, 2004·Retrovirology·Barbara AhrMartine Biard-Piechaczyk
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sayed F AbdelwahabGeorge K Lewis
Jul 27, 1999·Gastroenterology·M B DwinellM F Kagnoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.