The CCL2 chemokine is a negative regulator of autophagy and necrosis in luminal B breast cancer cells

Breast Cancer Research and Treatment
Wei Bin FangNikki Cheng

Abstract

Luminal A and B breast cancers are the most prevalent forms of breast cancer diagnosed in women. Compared to luminal A breast cancer patients, patients with luminal B breast cancers experience increased disease recurrence and lower overall survival. The mechanisms that regulate the luminal B subtype remain poorly understood. The chemokine CCL2 is overexpressed in breast cancer, correlating with poor patient prognosis. The purpose of this study was to determine the role of CCL2 expression in luminal B breast cancer cells. Breast tissues, MMTV-PyVmT and MMTV-Neu transgenic mammary tumors forming luminal B-like lesions, were immunostained for CCL2 expression. To determine the role of CCL2 in breast cancer cells, CCL2 gene expression was silenced in mammary tumor tissues and cells using TAT cell-penetrating peptides non-covalently cross linked to siRNAs (Ca-TAT/siRNA). CCL2 expression was examined by ELISA and flow cytometry. Cell growth and survival were analyzed by flow cytometry, immunocytochemistry, and fluorescence microscopy. CCL2 expression was significantly increased in luminal B breast tumors, MMTV- PyVmT and MMTV-Neu mammary tumors, compared or normal breast tissue or luminal A breast tumors. Ca-TAT delivery of CCL2 siRNA...Continue Reading

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Citations

Apr 30, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Min YaoNikki Cheng
Jul 26, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monoranjan Boro, Kithiganahalli Narayanaswamy Balaji
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May 30, 2020·Seminars in Cancer Biology·Haseeb ZubairAjay Pratap Singh
Dec 29, 2020·Frontiers in Oncology·Daniel Peña-OyarzúnAlfredo Criollo
Mar 3, 2021·Molecular Nutrition & Food Research·Alana Carolina Costa VerasMarcio Alberto Torsoni
May 12, 2021·Cancer Metastasis Reviews·Élora MidavainePhilippe Sarret

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