The CcrM DNA methyltransferase of Agrobacterium tumefaciens is essential, and its activity is cell cycle regulated

Journal of Bacteriology
L S Kahng, Lucy Shapiro

Abstract

DNA methylation is now recognized as a regulator of multiple bacterial cellular processes. CcrM is a DNA adenine methyltransferase found in the alpha subdivision of the proteobacteria. Like the Dam enzyme, which is found primarily in Escherichia coli and other gamma proteobacteria, it does not appear to be part of a DNA restriction-modification system. The CcrM homolog of Agrobacterium tumefaciens was found to be essential for viability. Overexpression of CcrM is associated with significant abnormalities of cell morphology and DNA ploidy. Mapping of the transcriptional start site revealed a conserved binding motif for the global response regulator CtrA at the -35 position; this motif was footprinted by purified Caulobacter crescentus CtrA protein in its phosphorylated state. We have succeeded in isolating synchronized populations of Agrobacterium cells and analyzing their progression through the cell cycle. We demonstrate that DNA replication and cell division can be followed in an orderly manner and that flagellin expression is cyclic, consistent with our observation that motility varies during the cell cycle. Using these synchronized populations, we show that CcrM methylation of the chromosome is restricted to the late S phas...Continue Reading

References

Oct 1, 1977·Journal of Bacteriology·M Evinger, N Agabian
Jan 1, 1991·Methods in Enzymology·G A CangelosiE W Nester
May 25, 1991·Nucleic Acids Research·G G Wilson
Jan 14, 1994·Journal of Molecular Biology·G ZweigerL Shapiro
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·C StephensL Shapiro
Aug 1, 1997·Trends in Genetics : TIG·R Jaenisch
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·K C QuonG T Marczynski
Aug 28, 1999·Journal of Bacteriology·A ReisenauerL Shapiro
Aug 28, 1999·Journal of Bacteriology·B W GoodnerG Grabowski
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·F García-Del PortilloJ Casadesús
Dec 10, 1999·Cell·A P Bird, A P Wolffe
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A S Lang, J T Beatty
Feb 26, 2000·Molecular Microbiology·B Nicholson, D Low
Jun 27, 2000·Journal of Bacteriology·J ZhuS C Winans
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D M HeithoffM J Mahan
Sep 20, 2000·Journal of Molecular Biology·M C Ouimet, G T Marczynski
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·W C NiermanJ Eisen

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Citations

Nov 5, 2002·Veterinary Microbiology·J-J LetessonX De Bolle
Mar 20, 2004·Nature Reviews. Microbiology·Jeffrey M Skerker, Michael T Laub
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Pamela J B BrownYves V Brun
Oct 9, 2007·Journal of Bacteriology·Anna SkoglundLars Engstrand
Jul 16, 2008·Journal of Bacteriology·Djenann Saint-DicLyn Sue Kahng
Dec 15, 2005·Journal of Bacteriology·Elizabeth J SummerRy Young
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josep Casadesús, David Low
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Patrick D Curtis, Yves V Brun
Feb 9, 2010·PloS One·Siu-Hong ChanShuang-Yong Xu
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jennifer B KozdonHarley H McAdams
Jan 9, 2014·Nucleic Acids Research·Diego GonzalezJustine Collier
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zachary E R NewbyThomas C Bruice
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Justine CollierLucy Shapiro
Jun 19, 2014·Nature Communications·Coralie FumeauxPatrick H Viollier
Jul 9, 2011·Seminars in Cell & Developmental Biology·Pamela J B BrownYves V Brun
Sep 29, 2009·Current Opinion in Microbiology·Justine Collier
Sep 2, 2009·Biochemical and Biophysical Research Communications·Hiroyuki IchidaTomoko Abe
Mar 4, 2009·Plasmid·Torsten Waldminghaus, Kirsten Skarstad
Apr 20, 2012·Molecular Microbiology·Patrick D CurtisYves V Brun
Jul 28, 2004·Trends in Microbiology·Régis HallezXavier De Bolle
Feb 25, 2006·Molecular and Biochemical Parasitology·Ohad FisherSerge Ankri
Apr 27, 2007·FEMS Microbiology Reviews·Jolanta Zakrzewska-CzerwińskaWalter Messer

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