The CD3gamma chain is essential for development of both the TCRalphabeta and TCRgammadelta lineages

The EMBO Journal
Mariëlle C HaksAda M Kruisbeek

Abstract

CD3gamma and CD3delta are the most closely related CD3 components, both of which participate in the TCRalphabeta-CD3 complex expressed on mature T cells. Interestingly, however, CD3delta does not appear to participate functionally in the pre-T-cell receptor (TCR) complex that is expressed on immature T cells: disruption of CD3delta gene expression has no effect on the developmental steps controlled by the pre-TCR. Here we report that in contrast with CD3delta, CD3gamma is an essential component of the pre-TCR. We generated mice selectively lacking expression of CD3gamma, in which expression of CD3delta, CD3epsilon, CD3zeta, pTalpha and TCRbeta remained undisturbed. Thus, all components for composing a pre-TCR are available, with the exception of CD3gamma. Nevertheless, T-cell development is severely inhibited in CD3gamma-deficient mice. The number of cells in the thymus is reduced to <1% of that in normal mice, and the large majority of thymocytes lack CD4 and CD8 and are arrested at the CD44-CD25+ double negative (DN) stage of development. Peripheral lymphoid organs are also practically devoid of T cells, with absolute numbers of peripheral T cells reduced to only 2-5% of those in normal mice. Both TCRalphabeta and TCRgammadel...Continue Reading

References

Mar 6, 1992·Cell·P MombaertsV E Papaioannou
Aug 1, 1991·The Journal of Experimental Medicine·P Pérez-AciegoJ R Regueiro
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J Kappes, S Tonegawa
Feb 1, 1990·European Journal of Immunology·F KoningJ E Coligan
Jan 1, 1990·Annual Review of Cell Biology·R D KlausnerJ S Bonifacino
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H SaitoC Terhorst
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H CleversC Terhorst
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J S BonifacinoR D Klausner
Feb 26, 1988·Science·A M WeissmanR D Klausner
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y MinamiR D Klausner
Oct 1, 1987·The Journal of Experimental Medicine·W G HaserS Tonegawa
Nov 11, 1994·Science·E W ShoresP E Love
Nov 1, 1995·The Journal of Experimental Medicine·N S van OersA Weiss
Nov 18, 1994·Science·C Saint-RufH von Boehmer
Apr 1, 1994·The Journal of Experimental Medicine·A WilsonH R MacDonald
Apr 1, 1994·European Journal of Immunology·H JacobsA Berns
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C N LeveltK Eichmann
Aug 1, 1993·International Immunology·F Lynch, E M Shevach
Oct 1, 1995·International Immunology·A Wilson, H R MacDonald
Feb 1, 1996·The Journal of Cell Biology·J DietrichC Geisler
Jan 1, 1996·Annual Review of Immunology·K Shortman, L Wu

❮ Previous
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Citations

Oct 25, 2003·Journal of Neuroimmunology·Alexandra WeberFrauke Zipp
Apr 17, 2002·Molecular Immunology·Timothy J Allison, David N Garboczi
Dec 15, 2000·Immunology Today·A M KruisbeekD L Wiest
Mar 14, 2000·Current Opinion in Immunology·V I Pivniouk, R S Geha
May 14, 1999·Current Opinion in Immunology·H von BoehmerO Azogui
Sep 11, 2002·Seminars in Immunology·Alison M Michie, Juan Carlos Zúñiga-Pflücker
Jul 14, 2000·Immunity·P E Love, E W Shores
Sep 17, 2009·The Journal of Biological Chemistry·Sun Taek KimEllis L Reinherz
Dec 10, 1999·The Journal of Experimental Medicine·A M MichieJ C Zúñiga-Pflücker
Sep 20, 2000·The Journal of Experimental Medicine·C L SommersP E Love
Jun 19, 2002·The Journal of Experimental Medicine·Maddalena PanigadaFabio Grassi
Jun 5, 2002·The Journal of Experimental Medicine·Tetsuro SasadaEllis L Reinherz
Jul 3, 2002·The Journal of Experimental Medicine·Mariëlle C HaksAda M Kruisbeek
Sep 29, 2004·The Journal of Experimental Medicine·Thijs J HagenbeekHergen Spits
Jan 19, 2006·The Journal of Experimental Medicine·Sandra M Hayes, Paul E Love
Jun 3, 2010·Cold Spring Harbor Perspectives in Biology·Paul E Love, Sandra M Hayes
Jul 22, 2005·Immunology and Cell Biology·Lisa A Miosge, Christopher C Goodnow
Dec 16, 1998·Immunological Reviews·D Amsen, A M Kruisbeek
Aug 14, 2003·Journal of Virology·Martha F KramerDonald M Coen
Jan 11, 2007·Molecular and Cellular Biology·Holger BabbeBoris Reizis
Jun 8, 1999·Annual Review of Immunology·W EllmeierD R Littman
Nov 5, 2002·Annual Review of Immunology·Timothy K StarrKristin A Hogquist
Mar 18, 2005·Annual Review of Immunology·Matthew E Call, Kai W Wucherpfennig
Nov 17, 2004·The Journal of Clinical Investigation·Geneviève de Saint BasileFrançoise Le Deist
Dec 12, 2012·PloS One·Safak Isil CevikBatu Erman
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J B DeJarnetteP E Love
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhen-Yu J SunGerhard Wagner
Mar 19, 2014·Immunology and Cell Biology·Yohei KawaiJi-Yang Wang
Jan 11, 2005·Current Opinion in Allergy and Clinical Immunology·Chaim M Roifman
Nov 3, 2005·Current Opinion in Allergy and Clinical Immunology·Alain FischerFrançoise Le Deist
Nov 17, 2009·Immunological Reviews·Vibhuti P Dave
Feb 13, 2007·Immunological Reviews·Sandra M Hayes, Paul E Love

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