Abstract
The role of CD40/CD40 ligand (CD40L) in microvascular thrombosis is now widely accepted. However, the exact mechanisms linking the CD40/CD40L system and the soluble form of CD40L (sCD40L) with microvascular thrombosis are currently a topic of intensive research. The objective of this study was to assess the potential mechanisms in CD40/CD40L system-regulated microvascular thrombosis after focal ischemia/reperfusion (I/R). Rats were subjected to 60-min transient middle cerebral artery occlusion (MCAO). The experiments were divided into three groups: sham operation, MCAO, and MCAO + CD40 antagonist. Dynamic changes of serum-free sCD40L levels for 0, 1, 3, 5, 6, and 12 h by ELISA detecting kit after focal I/R were observed, and the CD40 expression levels in both platelet surface and vascular endothelial cell surface were measured by flow cytometry and immunofluorescence, respectively. Cerebral infarct volume was analyzed 12 h after reperfusion. mTOR/S6K signaling was determined by Western blot. A comparison of thrombus formation between MCAO and CD40 antagonist treatment rats revealed a role for CD40 and/or CD40L in the inflammation-enhanced thrombosis responses in both of the platelet and vascular endothelial cell. MCAO rats yiel...Continue Reading
References
Jan 1, 1990·Clinical and Experimental Hypertension. Part A, Theory and Practice·T F Lüscher
Jan 1, 1993·Stroke; a Journal of Cerebral Circulation·T N LinC Y Hsu
May 31, 2003·Stroke; a Journal of Cerebral Circulation·Mami IshikawaD Neil Granger
Mar 30, 2005·Circulation·Mami IshikawaD Neil Granger
Dec 27, 2005·International Journal of Cardiology·Yoshitaka OhashiKojiro Awano
Jul 7, 2009·Neuromolecular Medicine·Gokhan Yilmaz, D Neil Granger
Dec 29, 2009·Frontiers in Bioscience (Landmark Edition)·Toni Kay RobertsJoan W Berman
Jan 7, 2014·Inflammatory Bowel Diseases·Serena L S YanD Neil Granger
Mar 25, 2014·Neuroscience Letters·Bikui ZhangRen Guo
Mar 26, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Chaolin QiuLimin Xu
May 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luokun XieKunlin Jin
Aug 29, 2014·Current Cancer Drug Targets·Gui Han LeeHafid Omar Al-Hassi
Aug 4, 2015·Journal of Autoimmunity·Hanna J LeeYaron Tomer
Jun 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabel VogelJan L Ceuppens
Jun 28, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daojing LiJunwei Hao
Citations
Jun 1, 2020·Journal of Molecular Neuroscience : MN·Chao LiuYupeng Wang
Jan 23, 2020·International Journal of Molecular Sciences·Cristina Barale, Isabella Russo
Sep 11, 2020·Oxidative Medicine and Cellular Longevity·Maciej BladowskiAdrian Doroszko
May 25, 2021·Journal of Cardiovascular Pharmacology·Ming YuanYongjun Yang