The CDC42 homolog of the dimorphic fungus Penicillium marneffei is required for correct cell polarization during growth but not development

Journal of Bacteriology
K J BoyceA Andrianopoulos

Abstract

The opportunistic human pathogenic fungus Penicillium marneffei is dimorphic and is thereby capable of growth either as filamentous multinucleate hyphae or as uninucleate yeast cells which divide by fission. The dimorphic switch is temperature dependent and requires regulated changes in morphology and cell shape. Cdc42p is a Rho family GTPase which in Saccharomyces cerevisiae is required for changes in polarized growth during mating and pseudohyphal development. Cdc42p homologs in higher organisms are also associated with changes in cell shape and polarity. We have cloned a highly conserved CDC42 homolog from P. marneffei named cflA. By the generation of dominant-negative and dominant-activated cflA transformants, we have shown that CflA initiates polarized growth and extension of the germ tube and subsequently maintains polarized growth in the vegetative mycelium. CflA is also required for polarization and determination of correct cell shape during yeast-like growth, and active CflA is required for the separation of yeast cells. However, correct cflA function is not required for dimorphic switching and does not appear to play a role during the generation of specialized structures during asexual development. In contrast, hetero...Continue Reading

References

Dec 1, 1975·Genetical Research·N R Morris
Sep 1, 1994·Mycopathologia·P Imwidthaya
Dec 1, 1994·Microbiology·N A Gow
Aug 1, 1994·Molecular and Cellular Biology·T Som, V S Kolaparthi
Feb 1, 1994·Molecular and Cellular Biology·P J Miller, D I Johnson
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·H U MöschG R Fink
Feb 7, 1998·Science·A Hall
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·F Banuett
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Schmidt, M N Hall
Nov 30, 1999·Molecular Cell Biology Research Communications : MCBRC·Y Lu, J Settleman
Jan 19, 2000·Molecular Biology of the Cell·K G KozminskiD G Drubin

❮ Previous
Next ❯

Citations

Dec 4, 2003·Fungal Genetics and Biology : FG & B·Gretel M GuestMichelle Momany
Mar 5, 2004·Fungal Genetics and Biology : FG & B·Steven D Harris, Michelle Momany
Nov 30, 2002·Current Opinion in Microbiology·Michelle Momany
Aug 26, 2003·Molecular Biology of the Cell·Philipp KnechtlePeter Philippsen
May 25, 2004·Molecular Biology of the Cell·Claire L PearsonSteven D Harris
Jan 19, 2006·Clinical Microbiology Reviews·Nongnuch VanittanakomThira Sirisanthana
Nov 29, 2002·Eukaryotic Cell·Brian D ShawMichelle Momany
Nov 10, 2005·Eukaryotic Cell·Carolyn G Rasmussen, N Louise Glass
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Katherine A BorkovichRobert Pratt
Nov 7, 2007·PLoS Pathogens·Kylie J Boyce, Alex Andrianopoulos
Jan 31, 2008·Future Microbiology·Chester R Cooper, Nongnuch Vanittanakom
Jun 7, 2011·Trends in Microbiology·Kylie J Boyce, Alex Andrianopoulos
Aug 19, 2009·Fungal Genetics and Biology : FG & B·A J AlmeidaF Rodrigues
Mar 21, 2009·Fungal Genetics and Biology : FG & B·Wu ZhengZonghua Wang
Feb 1, 2008·Fungal Genetics and Biology : FG & B·Abigail C Leeder, Geoffrey Turner
Nov 17, 2007·Molecular Microbiology·Aleksandra ViragSteven D Harris
May 10, 2013·Molecular Microbiology·Hayley E BugejaAlex Andrianopoulos
Feb 23, 2011·Molecular Microbiology·Steven D Harris
Oct 18, 2005·Fungal Genetics and Biology : FG & B·Supinya PongsunkSansanee C Chaiyaroj
Apr 14, 2016·Future Microbiology·Harshini WeerasingheAlex Andrianopoulos
Aug 16, 2011·Fungal Genetics and Biology : FG & B·Leonie KokkelinkPaul Tudzynski
Jun 12, 2013·Fungal Genetics and Biology : FG & B·Elizabeth Ripley BallouJ Andrew Alspaugh
Aug 31, 2006·International Review of Cytology·Steven D Harris
May 24, 2012·Biotechnology & Genetic Engineering Reviews·Marjatta RaudaskoskiJ Stephen Horton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.