The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop (Argopecten irradians, Lamarck 1819) responding to bacteria challenge and naphthalin stress

Fish & Shellfish Immunology
Linsheng SongKezhi Xing

Abstract

Heat shock protein 70 (HSP70) is an important member of the heat shock protein superfamily, and it plays a key role in the process of protecting cells, facilitating the folding of nascent peptides and responding to stress. The cDNA of bay scallop Argopecten irradians HSP70 (designated AIHSP70) was cloned by the techniques of homological cloning and rapid amplification of cDNA end (RACE). The full length of AIHSP70 cDNA was 2651bp in length, having a 5' untranslated region (UTR) of 96bp, a 3' UTR of 575bp, and an open reading frame (ORF) of 1980bp encoding a polypeptide of 659 amino acids with an estimated molecular mass of 71.80kDa and an estimated isoelectric point of 5.26. BLAST analysis revealed that the AIHSP70 gene shared high identity with other known HSP70 genes. Three classical HSP signature motifs were detected in AIHSP70 by InterPro analysis. 3-D structural prediction of AIHSP70 showed that its N terminal ATPase activity domain and C terminal substrate-binding domain shared high similarity with that in human heat shock protein 70. The results indicated that the AIHSP70 was a member of the heat shock protein 70 family. A semi-quantitive RT-PCR method was used to analyse the expression of AIHSP70 gene after the treatmen...Continue Reading

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Citations

Jan 29, 2011·BMC Genomics·Paola VenierAlberto Pallavicini
Mar 2, 2013·PloS One·Mauro de Freitas RebeloSilvana Allodi
Jan 26, 2016·Fish & Shellfish Immunology·Xiaorui SongLinsheng Song
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Nov 1, 2011·Ecotoxicology and Environmental Safety·Nik VeldhoenCaren C Helbing
Jun 2, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wei Xu, Mohamed Faisal
May 12, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Daxian ZhaoJianguang Qin
Dec 17, 2008·Fish & Shellfish Immunology·M Prado-AlvarezA Figueras
Jun 8, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qiang GaoYaqing Chang
Apr 15, 2015·Environmental Science and Pollution Research International·Fabrício Flores-NunesAfonso C D Bainy

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